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Products
The QIAsymphony DNA Maxi Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
QIAsymphony DNA Maxi Kit (96)
Cat. No. / ID. / ID. 937266
For 96 preps of 4 ml each: 2 reagent cartridges, 2 piercing lids, 2 reuse seal sets, buffers and Proteinase K solution
Features
- Process 24 samples in 4.75 hours
- Reduce risk of human error with prefilled reagent cartridges
- Automate tracking of samples and reagents using a bar code system
- Get highly intact DNA regardless of stabilization method
Product Details
Automate the purification of high-quality gDNA from human whole blood samples (up to 4 ml) using the QIAsymphony SP.
Performance
Automated DNA purification from 4 ml on the QIAsymphony SP results in high yields (measured via gel electrophoresis and spectrophotometer) of high-molecular-weight DNA (see High-molecular-weight DNA) in 4.75 hours (see QIAsymphony DNA Maxi Kit workflow), and even faster for 2 ml or for fewer than 24 samples.
See figures
Principle
The QIAsymphony DNA Maxi Kit uses an interlaced system (see QIAsymphony DNA Maxi Kit workflow) that shortens the time needed to complete the process:
- The QIAsymphony SP takes 1 ml from the 4 ml sample and lyses that portion.
- While the first 1 ml is in the bind process, a second 1 ml is taken from the sample and gets lysed simultaneously.
- DNA from this 1 ml is bound with the same magnetic beads that were used for the first 1 ml. While this is happening, the third 1 ml is being lysed.
- DNA from the third 1 ml is bound in the same magnetic beads used with the previous portions. At the same time, the fourth 1 ml is being lysed.
- DNA from the last 1 ml portion is bound like the others.
See figures
Procedure
The QIAsymphony SP performs all 4 steps (lyse, bind, wash and elute) of the purification procedure for up to 36 samples in a single run.
Supporting data and figures
QIAsymphony DNA Maxi Kit workflow.
Sample is split into four parts for faster processing.
Specifications
| Features | Specifications |
|---|---|
| Applications | PCR, real-time PCR, genotyping |
| Elution volume | 200–500 µl |
| Main sample type | Human whole blood |
| Sample amount | 2 or 4 ml (smaller volumes can be adjusted with PBS to 2 or 4 ml) |
| Yield | 60–150 µg from 4 ml sample (on average; samples with particularly high or low WBC count may fall outside that range) |
Resources
Safety Data Sheets (2)
Application/Protocol Documents (2)
Protocol Files (1)
Labware Documents (1)
Kit Handbooks (1)
Certificates of Analysis (1)