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When performing PCR, your experiments’ success can be impacted by a number of critical factors including the throughput, assay sensitivity, robustness of the reaction even with difficult templates, and reliable data normalization. Difficult templates with a high GC content, low template amounts and the need to optimize reaction conditions pose additional challenges.
Our PCR reagents and PCR enzymes guide your success and help you overcome the challenges you experience. The dual-cation PCR buffer in our PCR kits maintains a high primer annealing specificity over a broad range of temperatures which means you don’t have to optimize the PCR for each individual primer–template system.