Isolation of plasmid DNA from Staphylococcus spp. using the QIAGEN® Plasmid Midi Kit - (EN)

The procedure has been used successfully for isolation of a variety of medium-copy-number shuttle vectors from S. xylosus, S. carnosus, S. epidermidis, and S. aureus. Yield of plasmid DNA was typically 2-10 µg from 50 ml culture.