Isolation of plasmid DNA from Lactobacillus spp. using the QIAGEN® Plasmid Midi Kit - (EN)

The procedure has been used successfully for isolation of cryptic plasmids (pLC2-based) from mesophilic Lactobacillus strains such as L. sake and L. curvatus. Yield of plasmid DNA was typically 10-20 µg plasmid DNA from 100 ml culture.