Fast-forward protocol for transient transfection of 293 cells in 96-well plates using Effectene® Transfection Reagent - (EN)

The following protocol is optimized for transient transfection of 293 cells in 96-well plates without pre-plating of cells 24 h prior to transfection. Cell plating and transfection are performed on the same day, making this protocol rapid and convenient.