| Cell Line Species/Tissue: |
Drosophila melanogaster
/
|
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
Shields + Sang M3 Insect Medium + Pen/Strep |
Percent Serum (%):
|
12.5 |
Reporter System:
|
Luciferase |
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
T25 |
Number of Cells:
|
|
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
2 |
Amount of Enhancer (µl):
|
16 |
Amount of Reagent (µl):
|
20 |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
|
Transfection Efficiency (%):
|
110,000 RLU |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| Mosher, J.T., and Crews, S.T. (1999) Effectene™ Reagent yields high transfection efficiencies with Drosophila melanogaster S2 cells. QIAGEN News 1999, No. 4 |