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Transfection Cell Database - Detailed View


Cell Line: 3 B/2

Cell Line Species/Tissue: Opossum / Kidney proximal tubulus 
Transfection Reagent: Effectene 
Nucleic Acid:
DNA 
Growth Medium:
DMEM/F12 (1:1) with Glutamax-I (Gibco); 20 mM HEPES pH 7.4; 50 IU/ml penicillin; 50 ug/ml streptomycin 
Percent Serum (%):
10 FCS 
Reporter System:
 
Plasmid Purification Method:
QIAfilter Plasmid Kit 
Plate Format:
12-well plate 
Number of Cells:
456000 
Percent Confluence(%):
60 
Amount of Nucleic Acid (µg):
0.3 
Amount of Enhancer (µl):
2.4 
Amount of Reagent (µl):
18 
Complex Incubation on Cells (hrs):
24 
Analysis Performed Post-Transfection (hrs):
120 
Transfection Efficiency (%):
90% 
 
Any modifications to the protocol?:
 
Notes:
 
References: