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Transfection Cell Database - Detailed View


Cell Line: 293

Cell Line Species/Tissue: Human / Kidney Epithelial 
Transfection Reagent: Effectene 
Nucleic Acid:
DNA 
Growth Medium:
DMEM 
Percent Serum (%):
10 
Reporter System:
 
Plasmid Purification Method:
QIAGEN Plasmid Kit 
Plate Format:
 
Number of Cells:
 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
 
Amount of Enhancer (µl):
16 
Amount of Reagent (µl):
25 
Complex Incubation on Cells (hrs):
 
Analysis Performed Post-Transfection (hrs):
 
Transfection Efficiency (%):
 
 
Any modifications to the protocol?:
 
Notes:
cDNAs cloned into the CMV-driven pcDNA3.1/GS vector. Alternative to protein microarrays for the identification of drug targets and an expression cloning system for the discovery of gene products that alter cellular physiology  
References:
Microarrays of cells expressing defined cDNAs, Junaid Ziauddin & David M. Sabatini, Nature/Vol 411/3 May 2001