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Transfection Cell Database - Detailed View


Cell Line: 293

Cell Line Species/Tissue: Human / Kidney Epithelial 
Transfection Reagent: PolyFect 
Nucleic Acid:
DNA 
Growth Medium:
DMEM w/antibiotics 
Percent Serum (%):
10 
Reporter System:
 
Plasmid Purification Method:
 
Plate Format:
 
Number of Cells:
 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
0.5 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
Complex Incubation on Cells (hrs):
~16 
Analysis Performed Post-Transfection (hrs):
72-96 
Transfection Efficiency (%):
 
 
Any modifications to the protocol?:
Plated 150,000 cells per well 24 hours before transfection. Dilute 0.5 ug of plasmid DNA into 50 ul of serum free media before adding reagent.  
Notes:
Best conditions found for transfection of 293 cells in 24-well plate. Perfomed proprietary enzyme assay to evaluate results  
References: