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Transfection Cell Database - Detailed View
| Cell Line Species/Tissue: |
Canine
/
Kidney Epithelial |
| Transfection Reagent: |
PolyFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM |
Percent Serum (%):
|
10 |
Reporter System:
|
GFP |
Plasmid Purification Method:
|
HiSpeed Plasmid Kit |
Plate Format:
|
30mm dish |
Number of Cells:
|
200,000 |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
1.4 µl |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
10 µl |
Complex Incubation on Cells (hrs):
|
36h |
Analysis Performed Post-Transfection (hrs):
|
36h |
Transfection Efficiency (%):
|
20-30% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Also effective for stable transfection |
| References: |
| |
|