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Transfection Cell Database - Detailed View


Cell Line: 293T

Cell Line Species/Tissue: Human / Embryo kidney 
Transfection Reagent: TransMessenger 
Nucleic Acid:
siRNA (dsRNA) 
Growth Medium:
DMEM +10% FBS + Pen/Strep 
Percent Serum (%):
No serum 
Reporter System:
 
Plasmid Purification Method:
 
Plate Format:
24-well 
Number of Cells:
5x10e5/well 
Percent Confluence(%):
~60% 
Amount of Nucleic Acid (µg):
0.8 µg 
Amount of Enhancer (µl):
1.6 µg 
Amount of Reagent (µl):
4 µl 
Complex Incubation on Cells (hrs):
3 h 
Analysis Performed Post-Transfection (hrs):
24-48 h 
Transfection Efficiency (%):
~90% 
Knockdown Efficiency (%):  
 
Any modifications to the protocol?:
none  
Notes:
Compared TKO, Oligofectamine, and TransMessenger in parallel experiments. TKO: 30% Oligofectamine: 50% TransMessenger: 90%  
References: