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Transfection Cell Database - Detailed View
| Cell Line: Primary Bone-Marrow Stem Cells
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| Cell Line Species/Tissue: |
Mouse
/
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| Transfection Reagent: |
HiPerFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
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Percent Serum (%):
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Reporter System:
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Plasmid Purification Method:
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Plate Format:
|
6-well plate |
Number of Cells:
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Percent Confluence(%):
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n.d. |
Amount of Nucleic Acid (µg):
|
24 nM |
Amount of Enhancer (µl):
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Amount of Reagent (µl):
|
12 µl |
Complex Incubation on Cells (hrs):
|
24 hrs |
Analysis Performed Post-Transfection (hrs):
|
24 hrs |
Transfection Efficiency (%):
|
> 90% |
| Knockdown Efficiency (%): |
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| |
| Any modifications to the protocol?:
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| Notes: |
| Analysis by fluorescently labelled siRNA (Alexafluor 488). |
| References: |
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