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Transfection Cell Database - Detailed View
| Cell Line: Choroid plexus
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| Cell Line Species/Tissue: |
adult male Sprague-Dawley rats
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| Transfection Reagent: |
Effectene |
Nucleic Acid:
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DNA |
Growth Medium:
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Eagle’s modified essential medium, 5 units/ml penicillin, and 5 µg/ml streptomycin |
Percent Serum (%):
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10% fetral bovine |
Reporter System:
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Plasmid Purification Method:
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Plate Format:
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glass-bottomed confocal chambers (~4.9 cm2) |
Number of Cells:
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no information |
Percent Confluence(%):
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no information |
Amount of Nucleic Acid (µg):
|
0.5-1 µg |
Amount of Enhancer (µl):
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no information |
Amount of Reagent (µl):
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no information |
Complex Incubation on Cells (hrs):
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Analysis Performed Post-Transfection (hrs):
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Transfection Efficiency (%):
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| Any modifications to the protocol?:
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| Notes: |
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| References: |
| Ventricular choline transport. A role for organic cation transporter 2 expressed in choroid plexus
Sweet, D.H., Miller, D.S. and Pritchard, J.B.
J. Biol. Chem. (2001) 276(45), 41611-41619 |
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