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Transfection Cell Database - Detailed View
| Cell Line Species/Tissue: |
Human/Melanoma
/
|
| Transfection Reagent: |
Attractene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM |
Percent Serum (%):
|
|
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
24-well |
Number of Cells:
|
Not supplied |
Percent Confluence(%):
|
Not supplied |
Amount of Nucleic Acid (µg):
|
0.4 µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
3 µl |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
|
Transfection Efficiency (%):
|
25-40% depending on the plasmid transfected |
| |
| Any modifications to the protocol?:
|
| No |
| Notes: |
| No toxicity was observed. Several ratios of plasmid DNA to reagent were tested as detailed in the handbook. All ratios worked, and 0.4 µg DNA plus 3 µl reagent provided optimal results in 24-well plates. |
| References: |
| |
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