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Transfection Cell Database - Detailed View


Cell Line: A375

Cell Line Species/Tissue: Human/Melanoma /  
Transfection Reagent: Attractene 
Nucleic Acid:
DNA 
Growth Medium:
DMEM 
Percent Serum (%):
 
Reporter System:
 
Plasmid Purification Method:
 
Plate Format:
24-well 
Number of Cells:
Not supplied 
Percent Confluence(%):
Not supplied 
Amount of Nucleic Acid (µg):
0.4 µg 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
3 µl 
Complex Incubation on Cells (hrs):
 
Analysis Performed Post-Transfection (hrs):
 
Transfection Efficiency (%):
25-40% depending on the plasmid transfected 
 
Any modifications to the protocol?:
No  
Notes:
No toxicity was observed. Several ratios of plasmid DNA to reagent were tested as detailed in the handbook. All ratios worked, and 0.4 µg DNA plus 3 µl reagent provided optimal results in 24-well plates.  
References: