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Transfection Cell Database - Detailed View
| Cell Line Species/Tissue: |
Human
/
Prostate Carcinoma |
| Transfection Reagent: |
Attractene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
|
Percent Serum (%):
|
|
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
24-well |
Number of Cells:
|
|
Percent Confluence(%):
|
80% |
Amount of Nucleic Acid (µg):
|
1 µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
1,5 µl |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
|
Transfection Efficiency (%):
|
|
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Customer compared Attractene in 3 different prostate cancer cells to FuGeneHD: 22Rv1, LNCaP and PC3
Worked 10x better than FuGene HD.
Co- transfection of different plasmids. |
| References: |
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