Le site web n’est pas disponible en français dans son intégralité. Son contenu est international et peut aller à l’encontre des règlementations locales.
Transfection Cell Database - Detailed View
| Cell Line Species/Tissue: |
Human
/
|
| Transfection Reagent: |
Attractene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
MEM +FBS, L-glutamine, sodium pyruvate |
Percent Serum (%):
|
10% |
Reporter System:
|
Western Blot anti-FLAG |
Plasmid Purification Method:
|
Endofree Plasmid Kit |
Plate Format:
|
6-well plate |
Number of Cells:
|
5E5/well |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
2 |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
2.25 |
Complex Incubation on Cells (hrs):
|
24 |
Analysis Performed Post-Transfection (hrs):
|
0 |
Transfection Efficiency (%):
|
n/a |
| |
| Any modifications to the protocol?:
|
| No modifications. |
| Notes: |
| Liked: Adding DNA with transfection complex on the day of seeding.
Compared to FuGene and found that Attractene worked better. |
| References: |
| |
|