FAQ-2523
Is a special processing protocol needed?
To prevent biomolecule degradation during processing, dehydration must begin with at least 70–100% ethanol. We recommend using low-melting paraffin (melting point ≤56°C) and do not incubate samples in liquid paraffin for more than 3 hours.
Processing protocols for the PAXgene Tissue System are listed in the appendices of the PAXgene Tissue Container Product Circular and PAXgene Tissue FIX Container (50 ml) Product Circular.Related products
PAXgene Tissue RNA/miRNA Kit
Tissue samples fixed and stored in PAXgene Tissue Containers can be paraffin-embedded and used for pathological studies as well as for subsequent purification of miRNA, RNA, and/or DNA. The PAXgene Tissue miRNA Kit provides purification of total RNA, including RNA from approximately 18 nucleotides, from tissues fixed and stabilized in PAXgene Tissue Containers. Purification is carried out using silica-based RNA purification technology in a spin-column format. Used with the containers, the kit provides a complete preanalytical solution for collection, fixation, and stabilization through to purification of high-quality miRNA and total RNA for molecular analysis.
PAXgene Tissue FIX Container (50 ml)
The PAXgene Tissue FIX Container (50 ml) enables fixation and stabilization of tissue specimens (up to 4 samples with a maximum size of 4 x 15 x 15 mm placed into 4 standard tissue cassettes, or a single tissue sample with a maximum size of 20 x 20 x 20 mm). PAXgene Tissue FIX Containers (50 ml) are single-chamber containers prefilled with 50 ml of PAXgene Tissue FIX. PAXgene Tissue FIX rapidly penetrates and fixes the tissue, preserving tissue morphology. Fixation is comparable to formalin fixation, but without the destructive nucleic acid crosslinking and degradation. After fixation, PAXgene Tissue FIX is removed and replaced by PAXgene Tissue STABILIZER within the same container. Nucleic acids, proteins, and morphology of the sample are stable up to 7 days at room temperature, and for longer periods at 2–8°C, or even at –20°C and –80°C. Stabilized samples can be embedded in paraffin for histological studies. PAXgene Tissue Kits and supplementary protocols provide efficient subsequent purification of RNA, miRNA, DNA, and/or proteins from the same sample. PAXgene Tissue FIX Container (50 ml) is only to be used in conjunction with PAXgene Tissue STABILIZER.