| Cell Line Species/Tissue: |
Mouse
/
|
| Transfection Reagent: |
HiPerFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
|
Percent Serum (%):
|
|
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
6-well plate |
Number of Cells:
|
|
Percent Confluence(%):
|
n.d. |
Amount of Nucleic Acid (µg):
|
24 nM |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
12 µl |
Complex Incubation on Cells (hrs):
|
24 hrs |
Analysis Performed Post-Transfection (hrs):
|
24 hrs |
Transfection Efficiency (%):
|
> 90% |
| Knockdown Efficiency (%): |
|
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Analysis by fluorescently labelled siRNA (Alexafluor 488). |
| References: |
| |