EZ1&2 ccfDNA Kit

For automated isolation of cell-free (cfDNA) DNA from human plasma or serum on EZ1 Advanced XL or EZ2 Connect

S_1084_5_GEN_V2

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

EZ2 Connect Tip Rack, Large Volume

Cat. No. / ID:   9027011

Rack for use with EZ2 Connect; for Large-Volume Tubes
HK$12,090.00
Log in To see your account pricing.
AccessoriesKitCard
EZ2 Connect Tip Rack - Large Volume
EZ1&2 ccfDNA Kit
Accessories
EZ1 Card
EZ2 Connect Accessories are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
The EZ1&2 ccfDNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • Fully automated cfDNA purification from up to 8 ml of plasma or serum​
  • Prefilled cartridges to ensure standardization and reduce human error​
  • High-quality cfDNA for downstream technologies like dPCR
  • Run times from 35 to 70 minutes (1–8 ml plasma)​

Product Details

The EZ1&2 ccfDNA Kit provides easy and fully automated extraction of high-quality cfDNA from up to 8 ml of serum or plasma samples. Magnetic-bead technology allows parallel processing of 14 samples on the EZ1 Advanced XL or 24 samples on the EZ2 Connect.

Performance

The EZ1&2 ccfDNA Kit allows fully automated processing of up to 8 ml without manual pre-enrichment or preparation of plates or tubes – saving time and tedious manual steps (see figure  Large-volume processing). Whereas automated systems for cfDNA are only fully automated for processing 1 ml plasma, the EZ1&2 ccfDNA Kit is designed for fully automated processing of any starting volume up to 8 ml plasma (see figure  Higher degree of automation).

Compared to kits from alternative suppliers, the EZ1&2 ccfDNA Kit allows faster extraction of cfDNA from the same sample volumes (see figure  Faster processing). Great linearity of cfDNA yields is achieved throughout the entire input range from 0.5 to 8 ml of plasma and  cDNA (see figure  Linear DNA yields).

See figures

Principle

Cell-free DNA, such as tumor-specific extracellular DNA fragments in the blood or fetal DNA in maternal blood, is present in serum or plasma usually as short fragments of <1000 bp. EZ1&2 ccfDNA Kits enable efficient purification of these circulating DNAs from human plasma or serum using proven magnetic bead techology.

Procedure

The EZ1&2 ccfDNA procedure is fully automated. Choose between the protocols for 2, 4 or 8 ml; prepare the sample tubes, place them and the EZ1&2 Cartridge into the machine and walk away. With the EZ1&2 ccfDNA Kit, 14 (EZ1 Advanced XL) or 24 (EZ2 Connect) ccfDNA samples can be processed in just 35 minutes (2 ml ​input volume), 46 minutes (4 ml input volume) or 70 minutes (8 ml input volume).

To allow flexibility, different starting volumes of plasma can be processed in one run with the standardized protocols after addition of appropriate volumes of PBS (see figure  Flexible starting volumes).

See figures

Applications

cfDNA isolated using the easy-to-use and highly automated kit is ideal for analysis by downstream applications, including next-generation sequencing, qPCR and digital PCR (see figure  Comparable yields in dPCR).

The EZ1&2 ccfDNA Kit is highly suited for use in:

  • Cancer research
  • Non-invasive prenatal testing research (NIPT)
  • ​Transplantation research
  • cfDNA-based mutational and methylation analysis
  • ​Biomarker discovery
See figures

Supporting data and figures

Resources

Kit Handbooks (1)
Operating Software (2)
EZ2 Connect Software
SOFTWARE (123MB)
Version 3
Quick-Start Protocols (1)
Safety Data Sheets (1)
Certificates of Analysis (1)

FAQ

Can I use a different elution buffer?
No, the elution step is optimized for the buffer included in the kit.
FAQ ID - 3847
Can the kit be used on the EZ2 Connect?
Yes, the kit can be used on the EZ2 Connect. The following accessory is needed to run the kit on this instrument: 
  •  EZ2 Connect Tip Rack - Large Volume (cat. no. 9027011)
FAQ ID - 3845
Can the kit be used on the EZ1 Advanced XL?
Yes, the kit can be used on the EZ1 Advanced XL. The following accessories are needed to run the kit on this instrument: 
  • EZ1 Advanced XL Tip Rack - Large Volume (cat. no. 9027008)
  • EZ1 Advanced XL ccfDNA Card (cat. no. 9026964)


FAQ ID - 3844
What is the average amount of cfDNA present in 1 ml plasma?
According to an interview with Professor Dennis Lo published in QIAGEN News Molecular Diagnostics, Issue No. 5, 2002, healthy individuals have about 500–1000 genome equivalents (DNA) per milliliter of serum/plasma.
For cell-free-circulating DNA in plasma, the concentration can range from 1–100 ng/ml in healthy individuals.
FAQ ID - 3846
Frequently purchased together
7 mL Large-Volume Tubes (48)
CAT NO./ID 951954
7 mL Large-Volume Tubes (48)
Two bags of 24 large-volume tubes (7 mL)
HK$219.00
EZ1&2 ccfDNA Kit (48)
CAT NO./ID 954854
EZ1&2 ccfDNA Kit (48)
For 48 preps (2, 4, 8, or 10 ml (only on the EZ2 Connect): 48 reagent cartridges (EZ1&2 ccfDNA), Magnetic Bead Suspension, Elution Buffer, Large-Volume Tubes (7 ml), Disposable Tip Holders, Disposable Filter-Tips, Elution Tubes (1.5 ml)
HK$8,420.00
EZ2 Connect Cartridge Rack
CAT NO./ID 9027012
EZ2 Connect Cartridge Rack
Cartridge rack for EZ2 Connect, for use with prefilled kit cartridges
HK$18,120.00
EZ2 Connect Tip Rack
CAT NO./ID 9027009
EZ2 Connect Tip Rack
Tip Rack for EZ2 Connect, for use with screw-cap tubes
HK$9,320.00
Investigator Quantiplex Pro RGQ Kit (200)
CAT NO./ID 387316
Investigator Quantiplex Pro RGQ Kit (200)
For use on QIAGEN RotorGene Q Real-Time Systems: Quantiplex Pro RGQ Reaction Mix, Quantiplex Pro RGQ Primer Mix, Male Control DNA M1, QuantiTect Nucleic Acid Dilution Buffer
HK$9,410.00
EZ2 Connect Tip Rack - Flip Cap Tube
CAT NO./ID 9027010
EZ2 Connect Tip Rack - Flip Cap Tube
Tip Rack for EZ2 Connect, for use with flip-cap tubes
HK$9,410.00
Buffer MTL (54 ml)
CAT NO./ID 19112
Buffer MTL (54 ml)
Buffer MTL (54 ml)
HK$388.00
Buffer G2 (260 ml)
CAT NO./ID 1014636
Buffer G2 (260 ml)
260 ml Lysis Buffer
HK$931.00
12-Tube Magnet
CAT NO./ID 36912
12-Tube Magnet
Magnet for separating magnetic beads in 12 x 1.5 ml or 2 ml tubes
HK$4,500.00
Cooling Adapter, 2 ml, v2, Qsym
CAT NO./ID 9020674
Cooling Adapter, 2 ml, v2, Qsym
Cooling adapter for 2 ml screw-cap tubes; for use with the QIAsymphony SP/AS instruments (software version 3.1 or higher)
HK$10,190.00
EZ2 AllPrep DNA/RNA FFPE Kit (48)
CAT NO./ID 954734
EZ2 AllPrep DNA/RNA FFPE Kit (48)
For 48 preps: EZ2 AllPrep DNA/RNA FFPE cartridge, disposable filter-tips and tip-holders, tubes, Paraffin Removal Solution, Proteinase K, DNase, RNase-free water and buffers
HK$7,670.00
50 mL Tube Adapter Set
CAT NO./ID 11960
50 mL Tube Adapter Set
Two tube adapters for disruption in 50 mL bead tubes on a TissueLyser III only.
HK$11,960.00