| Cell Line Species/Tissue: |
human
/
T-cell leukemia |
| Transfection Reagent: |
SuperFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
RPMI 1640, 1% glutamine, 10% FCS |
Percent Serum (%):
|
10 |
Reporter System:
|
b-gal , pCMV-beta (ClonTech) |
Plasmid Purification Method:
|
EndoFree Plasmid Kit |
Plate Format:
|
96-well plate |
Number of Cells:
|
|
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
0.5 |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
2-4 |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
|
Transfection Efficiency (%):
|
20-40 beta-gal units/ml |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| |