| Cell Line Species/Tissue: |
Human
/
Hepatocarcinoma |
| Transfection Reagent: |
HiPerFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
RPMI |
Percent Serum (%):
|
10% FCS |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
24-well plate |
Number of Cells:
|
60.000 cells/well |
Percent Confluence(%):
|
~60-80% |
Amount of Nucleic Acid (µg):
|
1-10nM |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
3µl |
Complex Incubation on Cells (hrs):
|
48h |
Analysis Performed Post-Transfection (hrs):
|
48h |
Transfection Efficiency (%):
|
|
| Knockdown Efficiency (%): |
80-90% |
| |
| Any modifications to the protocol?:
|
| None |
| Notes: |
| qRT-PCR, QIAGEN MAPK1 siRNA, FastForward protocol works better |
| References: |
| |