| Cell Line Species/Tissue: |
Human
/
|
| Transfection Reagent: |
HiPerFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
DMEM |
Percent Serum (%):
|
10 % |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
6 mm dish |
Number of Cells:
|
1x10e5/ml |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
0.5 µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
20 µl |
Complex Incubation on Cells (hrs):
|
48 h |
Analysis Performed Post-Transfection (hrs):
|
48 h |
Transfection Efficiency (%):
|
|
| Knockdown Efficiency (%): |
25 % |
| |
| Any modifications to the protocol?:
|
| Yes |
| Notes: |
| using 2 times as much nucleic acid as standard
Western Blot, quantitative analysis |
| References: |
| |