FAQ-1575
Can samples in Allprotect Tissue Reagent undergo repeated freeze-thaws without degradation of DNA, RNA, and protein?
In general, yes. Freezing and thawing a sample incubated in Allprotect Tissue Reagent several times does not affect analysis of RNA and protein in downstream applications.
However, if you would like to perform protein activity assays, you should test each specific assay, since several freeze–thaw cycles might reduce protein activity, depending on the protein being assayed.
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Allprotect Tissue Reagent
Allprotect Tissue Reagent provides immediate stabilization of DNA, RNA, and protein in tissue samples at room temperature. This preserves the in vivo profile of DNA, RNA, and proteins, allowing reliable downstream analysis. Stabilized tissues can be transported at 15–25°C for up to 7 days, or stored at 2–8°C for up to 12 months. For longer storage, stabilized tissues can be archived at –20°C or –80°C.