| Cell Line Species/Tissue: |
Human
/
Monocyte |
| Transfection Reagent: |
HiPerFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
RPMI 1640; 10% FBS; 1X Pen-Strep; 1X Glutamine; 25mM B-Me |
Percent Serum (%):
|
|
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
100-mm dish |
Number of Cells:
|
5 X 10^6 |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
100nM |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
49 |
Complex Incubation on Cells (hrs):
|
36 |
Analysis Performed Post-Transfection (hrs):
|
|
Transfection Efficiency (%):
|
|
| Knockdown Efficiency (%): |
|
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| |