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RNeasy PowerBiofilm Kit (50)
Cat. No. / ID: 25000-50
For the isolation of high quality total RNA from biofilm samples
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This product contains substances regulated under REACH (EC 1907/2006 Annex XIV). The use of this product in the EU is permitted subject to an exemption (Article 56(3)). Please refer to the REACH notification and the SDS of this product, both of which can be found in the “Resources” section of this page, for more information.
The RNeasy PowerBiofilm Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- High-quality RNA isolation from all biofilms, challenging dental plaques and microbial mats
- High RNA yields with pre-treatment, mechanical and chemical lysis
- Efficient elimination of PCR inhibitors with Inhibitor Removal Technology
Product Details
Overcome inefficient cell lysis with the RNeasy PowerBiofilm Kit. Mechanical and chemical lysis are combined with Inhibitor Removal Technology to isolate pure RNA free from inhibitors typical of biofilms, including humic acid, metals, salts and pesticides. The resulting RNA is then ready for further analysis with downstream applications. Want to try this solution for the first time? Request a quote for a trial kit.
The RNeasy PowerBiofilm Kit was formerly sold by MO BIO as PowerBiofilm RNA Isolation Kit (50).
Supporting data and figures
Figure 1. High-quality RNA yield from a range of biofilms.
High-quality and yield RNA was recovered from samples regardless of biofilm origin. Several types of biofilms were collected and RNA isolated from those samples. Resulting RNA quality was assessed through gel electrophoresis; RNA yield was determined using NanoDrop.
*All yields represent combined results from multiple experiments and collaborators.
*All yields represent combined results from multiple experiments and collaborators.
Specifications
| Features | Specifications |
|---|---|
| Format | Silica Spin Filter Tubes |
| Sample size | 0.05 - 0.20 g |
| Throughput | 1-24 samples |
| Processing | Bead beating |
| Binding capacity | Up to 40 µg per prep |
| Time per run or per prep | 42 minutes (+15 minute DNase step) |
| Storage temperature | Store lyophilized DNase I at 4°C All other kit reagents and components should be stored at room temperature (15-30°C) |
| Bead size | 0.1 mm glass, 0.5 mm glass, 2.4 mm ceramic mix |
| Sample types | All biofilms from cultured single cell layers to microbial mats |
Resources
Safety Data Sheets (1)
Kit Handbooks (2)
Quick-Start Protocols (1)
Certificates of Analysis (1)