| Cell Line Species/Tissue: |
human
/
acute monocytic leukemia |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
RPMI |
Percent Serum (%):
|
|
Reporter System:
|
GFP |
Plasmid Purification Method:
|
|
Plate Format:
|
|
Number of Cells:
|
|
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
0.4 |
Amount of Enhancer (µl):
|
3.2 |
Amount of Reagent (µl):
|
10 |
Complex Incubation on Cells (hrs):
|
24 |
Analysis Performed Post-Transfection (hrs):
|
48 |
Transfection Efficiency (%):
|
13% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Cell density at plating = 2x10e7 cells/ml. |
| References: |
| |