QIAxcel DNA Kits

QIAxcelでDNAフラグメントの自動解析

✓ オンライン注文による24時間年中無休の自動処理システム

✓ 知識豊富で専門的な製品&テクニカルサポート

✓ 迅速で信頼性の高い（再）注文

QIAxcel DNA High Resolution Kit (1200)

Cat. No. / ID: 929002

QIAxcel DNA High Resolution Gel Cartridge, Buffers, Mineral Oil, QX Intensity Calibration Marker, 12-Tube Strips

￥154,000

Cartridge type

High Resolution

Screening

High Sensitivity

QIAxcel DNA Kitsは分子生物学的アプリケーション用であり、疾病の診断、予防、あるいは治療に使用することはできません。

✓ オンライン注文による24時間年中無休の自動処理システム

✓ 知識豊富で専門的な製品&テクニカルサポート

✓ 迅速で信頼性の高い（再）注文

特徴

即使用可能なゲルカートリッジで安全かつ簡便なシステム
能率的なワークフローで最小限の解析時間
わずか0.1 ng/μl の核酸濃度でも正確な結果
3～5 bpの高い分離能で正確な解析
デジタルデータ収集によりプロセスの標準化

製品詳細

QIAxcel Advanced System および QIAxcel System とQIAxcel DNA Kits との組み合わせで、1ランあたり最大96 サンプルからDNA フラグメントの分離および定量を自動的に行なえます。ゲルカートリッジを用いたキャピラリー電気泳動をベースにしたQIAxcel テクノロジーは、卓越した分離能、速度、スループット数を実現します。QIAxcel Gel Cartridges により12 サンプルの同時解析が可能です。QIAXcel Advancedにはほとんどのアプリケーションに最適なメソッドがインストール済みです。カスタマイズメソッドも作成できますので、テクニカルサポートにお問い合わせください。

パフォーマンス

高分離能解析を簡単に実現

生物のジェノタイピング、PCRによるcDNAライブラリースクリーニング、その他多くのバイオメディカル研究や分子生物学アプリケーションでは、PCRフラグメントのわずかな塩基対の長さの差を分析する必要があります。QIAxcel High Resolution KitとQIAxcel Advanced System および QIAxcel Systemの組み合わせで、15 bpから5 kbまでのDNAフラグメントの解析が可能です。500 bp未満のフラグメントは3～5 bpの分離能で分離され、従来の高分離能アガロースゲル電気泳動よりも信頼できるデータ解析が行なえます（図 "がん関連遺伝子の変異を高感度で検出"）。未希釈の増幅反応液のDNA検出感度は0.1 ng/μl で、QIAxcel Advanced System および QIAxcel Systemはわずかなサンプル量をインプットするだけで信頼できる結果が得られます。

QIAxcel DNA Screening Kit は、制限酵素分解におけるフラグメントのルーチン的な評価、シングルプレックスあるいはマルチプレックス定性PCRおよびその他のDNAアプリケーションに最適です（図 " 分離能の高いマルチプレックスPCR解析"）。DNAスクリーニング用ゲルカートリッジの使用により、15 bpから5 kbまでのサイズを持つDNAフラグメントの解析時間が短縮でき、96サンプルは約1時間で完了します。1 kb未満のフラグメントに関しては20～50 bpの分離能が得られます。本システムで使用するサンプル量は0.1 μl 以下なので、貴重なサンプルを他のアプリケーションのために残すことが可能です。

図参照

High-resolution analysis of multiplex PCR samples.

原理

QIAxcel Advanced System および QIAxcel Systemは、核酸の大きさにより迅速に核酸を分離するためにキャピラリーゲル電気泳動を用いています（図 " サンプル分離プロセス"）。従来のアガロースゲル電気泳動とは異なり、充填済みゲルカートリッジのキャピラリー内で分離が行なわれます。サンプルは自動的に個々のキャピラリーにアプライされ、電圧がかかります。負に電化した核酸はキャピラリー内を正に電化した末端まで移動します。アガロースゲル電気泳動と同様に、分子量の低い分子は高い分子よりも早く移動します。分子がキャピラリーを移動する際に検出器が蛍光シグナルを検出・測定します。光電子倍増管により蛍光シグナルが電子データに変換され、これがコンピューターにトランスファーされ、QIAxcel ScreenGel Software により処理されます。処理後、データはエレクトロフェログラムとゲルイメージの両方で表示されます。

図参照

Sample separation process.

操作手順

QIAxcel DNA Kits は、DNAフラグメントの分離と解析を簡素化し、ユーザーの安全性を高めます。操作は、少ないステップで簡単です。ゲルカートリッジをセット、バッファートレイの充填とセット、96ウェルプレート／PCR チューブ／ストリップにサンプルをアプライ、使用するprocess profileを選択します。煩わしいゲル作製や専門的なトレーニングは不要なので、ラボのワークフローが能率化され、ルーチンワークへのインテグレーションが容易に行なえます。泳動開始数分後には、コンピューターの画面に最初の結果がリアルタイムで表示されます（図 "ラン時間の短縮"）。

アプリケーション

プログラム済みのメソッドと対応するゲルカートリッジを組み合わせることにより、1個あるいは複数のPCRフラグメント、制限酵素反応により切断されたDNA、合成オリゴヌクレオチドなどを含む様々な核酸の分離と解析を実現します。

	QIAxcel DNA High Resolution Kit	QIAxcel DNA High Sensitivity Kit^§	QIAxcel DNA Screening Kit	QIAxcel DNA Fast Analysis Kit
Size range	15 bp – 20 kb; displaying up to 50 kb	15 bp – 3 kb	15 bp – 5 kb	15 bp – 3 kb
Resolution*	3–5 bp	20–50 bp	20–50 bp	50 bp
Limit of detection	0.1 ng/μl	5 pg/μl	0.1 ng/μl	0.1 ng/μl
Samples per cartridge	1200 samples	1200 samples	2400 samples	3000 samples
Run time^†	9–25 min	~9 min	~7 min	~3 min
Application area^‡	High-resolution genotyping VNTR, STRs, etc. gDNA integrity check NGS library check	Quality control of liquid biopsy extracts NGS library check	Fast PCR screening (single and multiplex) Restriction digest, plasmid gDNA integrity check NGS library check	Fast single amplicon analysis, e.g., HLA typing

STR: short tandem repeat; VNTR: variable number of tandem repeat.

* For 100–500 bp range.

^† For 12 samples.

^‡ Including, but not limited to.

^§ Only works in combination with the QIAxcel Connect System.

裏付けデータと数値

Reduced run times.

Nucleic acid separation on the QIAxcel system using the QIAxcel DNA High Resolution Kit takes less than 10% of the time of conventional high-resolution agarose.

Screenshot showing data from PCR fragment analysis.

Sensitive detection of a mutated cancer-related gene.

リソース

A protocol for evaluation of samples for next-generation sequencing on the QIAGEN GeneReader platform using the QIAxcel Advanced Instrument and ScreenGel software version 1.5 of higher

For evaluation of samples for next-generation sequencing (NGS) on the QIAGEN GeneReader platform using ScreenGel Software version 1.5 or higher

For evaluation of samples for next-generation sequencing (NGS) on the QIAGEN GeneReader instrument

For analysis and typing of PCR products from different HLA loci using the QIAxcel Advanced and the Helmberg-SCORE software

For automated analysis of DNA fragments using the QIAxcel and QIAxcel Advanced instruments

The QIAxcel System was successfully used to detect DNA derived from genetically modified organisms (GMOs) at a level suitable for GMO testing according to EU standards.

Download Safety Data Sheets for QIAGEN product components.

For electrophoretic analysis of DNA samples using the QIAxcel Connect system

This release includes the first version of QIAxcel ScreenGel NGS Profiles. These profiles are intended to be used to run and analyze reactions of GeneRead QIAact AIT and BRCA 1/2 panels using the QIAxcel instrument and QIAxcel ScreenGel 1.5 Software. These profiles are composed of an Process Profile, Analysis Profile, Distribution Profile, and Report/Export Profile.

For use with QIAxcel Advanced instruments and QIAxcel ScreenGel Software version 1.6

FAQ

The intensity calibration procedure on the QIAxcel System and QIAxcel Advanced is performed to normalize the signal intensities across all 12 channels of the gel cartridge. This corrects for natural intensity reading variations between each capillary in the cartridge. A correction factor is determined and applied for every subsequent run performed with the cartridge.

FAQ ID -1824

The QIAxcel DNA Fast Analysis Cartridge can only be run on the QIAxcel Fast Analysis System with the BioCalculator Fast Analysis software and the grey software key.

All other QIAxcel Cartridges can only be run on the QIAxcel System with the BioCalculator software and the blue software key.

FAQ ID -1828

The concentration of the DNA marker, pUC18/HaeIII, provided in the QIAxcel DNA Kits is 5ug/ml in 1XTE buffer solution. Customers can run their own DNA size marker sample with different concentration levels using the provided Methods (i.e. M500, H500, L500 and so on).

The recommended method for all basic applications with the QIAxcel System is the M500 method. The BioCalculator software also allows customers to choose their own Sample Injection Time in the Sequence table. The M500 method provides 5KV for 20 seconds for the electro-kinetic injection of lower concentrated DNA samples. If the Injection Time is doubled or tripled (40 or 60 sec), you can expect double or triple intensities (also peak heights).

FAQ ID -1841

For the BioCalculator software, the default data folder is:

C:\Program Files\QIAGEN\QIAxcel BioCalculator\Data.

For the ScreenGel software, the default data folders are:

C:\Documents and Settings\All Users\Application Data\QIAGEN\QIAxcel\ScreenGel\Data\Experiment (for a computer with Windows XP operation system) or C:\ProgramData\QIAGEN\QIAxcel\ScreenGel\Data\Experiment\ (for a computer with Windows 7 operating system).

Alternatively, you can more conveniently access the ScreenGel experiment data folder using the menu options File\Open data directory\Experiment within the ScreenGel software.

FAQ -9008

The Gel Cartridge does not need to be recalibrated when using a new alignment marker and/or DNA size marker with the QIAxcel System or QIAxcel Advanced. Calibration is required only once, i.e. when the first cartridge is run or when the cartridge is used on a different QIAxcel system than the one it has been calibrated on, or when using a different PC than the one used to calibrate initially.

FAQ ID -1858

If the gel cartridge tips for the QIAxcel System and QIAxcel Advanced are in contact with air, the tips will dry out. There are 3 ways of preventing the tips from drying.

Store the cartridge in the instrument with the instrument in the “Park” position. The washing solution (8 ml) covered with mineral oil must be in the “Park” position of the Solution Tray.
Store the cartridge in the Cartridge Stand with enough mineral oil in the well of the Cartridge Stand to cover the capillary tips. The Cartridge Stand should only be used for short term storage, i.e. when changing cartridges during one working day.
Place the cartridge back into its packaging with the capillary tips gently inserted into the soft gel in the box.

FAQ ID -1823

The reference size marker table created by ScreenGel version 1.1 or earlier is incompatible with ScreenGel version 1.2. Re-analyze the size marker run in ScreenGel version 1.2 and create a new reference size marker table for future use.

FAQ -9009

When using the BioCalculator software, ensure the color of the software key matches the type of cartridge used. For example, the QIAxcel Fast Analysis cartridge requires the red software key, while the QIAxcel High Resolution cartridge requires the blue software key.

In addition, check that the communication between the computer and the instrument is established. Further, check that the smart key is inserted securely.

If the issue persists, contact QIAGEN Technical Services and provide the cartridge ID and lot number, which is printed on the packaging of QIAxcel cartridges.

FAQ -9004

When using the BioCalculator software, select Settings under File in the Instrument control panel window. In the new windows, click Check Filter in the new window and follow the on-screen prompts.

When using the ScreenGel software, log in to the ScreenGel software with an account with basic or advanced privilege. Select System Check under the Service environment. Select Filter Check and click Start.

Please send the filter test result to QIAGEN Technical Services for further assistance.

FAQ -9006

Incorrect storage can lead to QX alignment marker degradation. The QX alignment markers can be used for 50 runs or 3 days, whichever comes first. Opened QX alignment marker should be store at –20°C between uses.

FAQ -9003

Clogged capillaries and/or re-using the calibration marker are the most common causes of failed calibration. Perform “Removing gel from blocked capillaries”, as outlined in Appendix D in the QIAxcel DNA handbook. Use a new set of calibration marker for each new calibration procedure.

FAQ -9001

Please delete the 6 obsolete method files as outlines in the Important Note QIAxcel ScreenGel Software

FAQ -9010

When using the BioCalculator software, select Detector test under File in the Instrument control panel window. Perform the Detector test with and without a cartridge latched inside the QIAxcel. Feedback is not shown on the screen when you select the Detect test. Instead, the file named detector.asc is saved at C:\Program Files\QIAGEN\QIAxcel BioCalculator. Change the file name before performing another detector test.

Note: Older versions of BioCalculator software save the detector.asc at C:\Program Files\QIAxcel BioCalculator.

When using the ScreenGel software, log in to the ScreenGel with an account with basic or advanced privilege. Select System Check under the Service environment. Select Detector Test and click Start. The file is saved at C:\Documents and Settings\All Users\Application Data\QIAGEN\QIAxcel\ScreenGel\Data\Troubleshooting\Troubleshooting_xxxxxxxx (current date in the format of YYYYMMDD) for a computer with Windows XP operating system or C:\ProgramData\QIAGEN\QIAxcel\ScreenGel\Data\Troubleshooting for a computer with Windows 7 operating system. Alternatively, you can perform a screen capture for the Detector test result.

Please send the Detector test results to QIAGEN Technical Services for further assistance.

FAQ -9006