EZ1 RNA Tissue Mini Kit

プレパック試薬カートリッジで高純度トータルRNA の自動精製

✓ オンライン注文による24時間年中無休の自動処理システム

✓ 知識豊富で専門的な製品&テクニカルサポート

✓ 迅速で信頼性の高い（再）注文

EZ1&2 RNA Tissue Mini Kit (48)

Cat. No. / ID: 959034

For 48 preps: Reagent Cartridges (Tissue RNA), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml), Buffer RLT, RNase-free DNase I, RNase-free water

￥60,000

The name of this kit was changed from "EZ1 RNA Tissue Mini Kit" to "EZ1&2 RNA Tissue Mini Kit" to demonstrate that it is compatible with the new EZ2 Connect instruments as well as the EZ1 Advanced XL. No changes were made to the well-established kit technology.

EZ1 RNA Tissue Mini Kitは分子生物学的アプリケーション用であり、疾病の診断、予防、あるいは治療に使用することはできません。

✓ オンライン注文による24時間年中無休の自動処理システム

✓ 知識豊富で専門的な製品&テクニカルサポート

✓ 迅速で信頼性の高い（再）注文

特徴

最大30 µgのトータルRNA精製
10 mg までの標準的な組織サンプルから信頼できる操作
RNaseフリーのセットアップで信頼できる結果
溶出容量が調節可能
プログラム済みのICカードを利用して簡単にプロトコールをセットアップ

製品詳細

EZ1 RNA Tissue Mini Kit には磁性粒子テクノロジーを用いて溶解しやすい組織サンプル（最高10 mg）からトータルRNAを自動精製するために必要な試薬やプラスチックウェアが全て入っています。プレパックカートリッジ試薬なので、ワークステーションへのセッティングも迅速かつ簡便に行なえます。EZ1 RNA Tissue Mini KitはEZ1 Advanced XLおよびEZ1 AdvancedあるいはBioRobot EZ1で使用可能です。

パフォーマンス

EZ1 RNA Tissue Mini Kit で精製したトータルRNAは、収量と高い品質が一定であって再現可能です（図" 効率的な精製および再現性の高い収量" と " 一定した高純度RNA"）。

図参照

Efficient purification and reproducible yields.

Consistent highly pure RNA.

原理

EZ1 RNA Tissue Mini Kitでの自動化精製には、EZ1 Advanced XLおよび EZ1 Advanced XL RNA Card、EZ1 Advancedおよび EZ1 Advanced RNA Card、あるいはBioRobot EZ1および EZ1 RNA Cardが必要です。EZ1 cardsにはオペレーターのためのスクリーン上での解説および精製操作用のコマンドが含まれています。

シリカゲルでコーティングされた磁性粒子テクノロジーにより、使いやすいワークステーションを用いて1回のランで1～14サンプルから（EZ1 Advanced XL）あるいは1～6サンプルから（EZ1 AdvancedおよびBioRobot EZ1）の精製が可能です。充填済みの試薬カートリッジとセンサーによるワークステーションドアの自動ロックにより、実験環境からのコンタミのリスクやRNaseによる分解を抑えることができます。自動化精製により再現性の高い結果と、広範囲にわたるスタートサンプル量から直線性のある収量が得られます。広範囲な組織タイプから高品質なトータルRNAを精製する柔軟性を持つため、様々な遺伝子発現解析アプリケーションのためのサンプルのRNA調製が可能です。EZ1 RNA Tissue Mini Kit を使用した場合、RNA収量は、サンプルの重量、組織の種類、細胞の発育状態などの多くの変数に左右されます。

操作手順

組織をまずBuffer RLTで溶解・ホモジナイズします。EZ1 RNA自動化操作に従いEZ1 AdvancedあるいはBioRobot EZ1でRNAがワンステップで精製されます。微量なDNAにも影響をうけるある種のRNAアプリケーションには、インテグレートされているDNase分解ステップによりゲノムDNAを除去します。トータルRNAは200 µl、100 µl、50 µl で溶出可能で、ダウンストリームアプリケーションに即使用可能です。

アプリケーション

EZ1 RNA Tissue Mini Kitを用いて得られた高純度RNAは、次のような多くのアプリケーションに最適です。

リアルタイムRT-PCRテクノロジーを用いた遺伝子発現解析
マイクロアレイテクノロジーを用いた遺伝子発現解析
腫瘍学研究

裏付けデータと数値

Efficient purification and reproducible yields.

Using the EZ1&2 RNA Tissue Mini procedure, total RNA was purified from 10 mg samples of rat kidney and liver and from 5 mg samples of rat pancreas, spleen, and lung. RNA was purified from 3 replicate samples of each tissue type. Yields of total RNA were determined by absorbance (A₂₆₀) with correction for background (A₃₂₀). CV kidney = 15%; CV liver = 1%; CV pancreas = 8%; CV spleen = 10%; CV lung = 22%.

Specifications

Features	Specifications
ApplicationsJA	PCR, real-time RT-PCR, microarray
Time per run or per prep	40 minutes
Technology	Magnetic particles
Elution volume	50–200 µl
Processing	Automated
Format	Sample tube
Stabilization	Yes
For automated processing	EZ2 Connect instruments or EZ1 Advanced XL
Main sample type	Tissue samples
Sample amount	5–10 µg
Yield	<30 µg
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	RNA

リソース

Fully automated low- to medium-throughput purification of nucleic acids

Download Safety Data Sheets for QIAGEN product components.

For purification of total RNA from tissue and cells using EZ1 instruments

FAQ

Operation of the BioRobot EZ1 is so easy that no additional training other than the initial demo is needed. Operating the instrument requires only three major steps:

Insert the appropriate EZ1 Protocol Card, switch on the workstation, and, if necessary, select the appropriate protocol
Follow the instructions on the liquid-crystal display (LCD), which describes where the sample tubes, elution tubes, filter tips, and EZ1 Reagent Cartridges must be loaded and which indicates when to start the protocol run
Collect purified nucleic acids when the protocol run ends

FAQ ID -1239

No, the new EZ1 RNA Kits can only be used with updated protocols programmed on the new EZ1 RNA Card V2.0.

The new card contains all protocols available for the isolation of RNA on the BioRobot EZ1 with the new kits:

Purification of total RNA from cells using the EZ1 RNA Cell Mini Kit, with and without DNase digest.
Purification of total RNA from standard tissue using the EZ1 RNA Tissue Mini Kit, with and without DNase digest.
Purification of total RNA from any type of tissue using the EZ1 RNA Universal Tissue Kit, with and without DNase digest.

Likewise, the former EZ1 RNA Cell Mini Kit (cat. no. 958134), EZ1 RNA Tissue Mini Kit (cat. no. 959134) and EZ1 RNA Universal Tissue Kit (cat. no. 956136) are not compatible with the new EZ1 RNA Card (cat. no. 9015590).

FAQ ID -1025

Yes, please follow the Supplementary Protocol 'Isolation of total nucleic acids from animal and human tissues using the EZ1 RNA Tissue Mini Kit'.

FAQ ID -975

Protocols for nucleic acid purification are supplied on pre-programmed EZ1 Protocol Cards. These protocols provide both on-screen instructions for the operator and operating commands for the workstation. The range of kits and protocols for the EZ1 is continuously expanding to allow nucleic acid purification from a wide range of sample types. Please browse our Supplementary Protocol Section to find specialized EZ1 protocols for a large variety of clinical sample types, in addition to the standard sample types covered in our EZ1 handbooks.

FAQ ID -1241

Loading of sample tubes, elution tubes, filter-tips, and Reagent Cartridges onto the worktable of the BioRobot EZ1 is intuitive, easy and quick. Details about worktable setup are provided with each new protocol, and are also displayed in the liquid-crystal display (LCD) of the control panel when you start setup of the worktable.

FAQ ID -1243

No, prefilled Reagent Cartridges can be loaded onto the BioRobot EZ1 with their seals. During operation of the instrument, the piercing unit, a row of 6 metal spikes located behind the tip adapters, will puncture the foil of the cartridges, thereby exposing the reagents.

FAQ ID -1240

For RNA isolated on the BioRobot EZ1 and BioRobot M48:

The RNA can be directly applied to the Agilent Bioanalyzer, since it is being denatured in the final protocol steps of these isolation procedures.

For RNA prepared with all other QIAGEN RNA Isolation Products:

We recommend to denature the samples in a water bath for 2 min at 70°C, and then place them directly on ice prior to loading them onto the Agilent Bioanalyzer.

FAQ ID -528

Yes, please follow the User-Developed Protocol 'Purification of total RNA from paraffin-embedded sections using the EZ1 RNA Tissue Mini Kit' (MA33).

FAQ ID -976

Yes, please follow the Supplementary Protocol 'Purification of DNA from fungi using the EZ1 DNA Tissue Kit' (MA35).

FAQ ID -992

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12

No extra accessories need to be purchased for the BioRobot EZ1, as sample and elution tubes, as well as tips and tip holders are included with each EZ1 DNA or RNA isolation kit.

FAQ ID -1246