Cat. No. / ID: 220211
QIAGEN OneStepAheadRT-PCR | Features | Benefits |
---|---|---|
Sensiscript and Omniscript reverse transcriptases | Wide range of RNA amounts (0.1 pg – 2 µg) High sensitivity Heat-mediated activation | Convenient room-temperature setup Applicable to automated workflows |
QuantiNova HotStarTaq, hot-start high-fidelity Polymerase | Highly specific products Heat-mediated activation Increased fidelity Short cycling time | Convenient room-temperature setup High sequence accuracy Amplification of long targets (<4 kb) Fast cycling time ~1 h |
Master mix with DNA polymerases, Buffers, dNTPs | Proprietary dual-cation buffer system Optimized for duplex PCR | Minimal optimization needed Convenient reaction setup No inhibition of PCR by reverse transcriptases Increased confidence due to optional inclusion of positive control with every reaction |
RNase inhibitor | Inhibition of RNases | RNA decay protection |
Q-Solution | Facilitates amplification of GC-rich templates | Better and more reliable performance |
Optional pipetting control | Inert orange and blue dyes Run-on gel at 50 bp (orange) and 4000 bp (blue) | Highest confidence by minimizing pipetting errors Also serves as a gel tracking dye |
Features | Specifications |
---|---|
ApplicationsJA | Gene expression analysis, virus detection |
Reaction type | One-step RT-PCR |
Enzyme activity | Reverse transcription, 5' -> 3' exonuclease activity |
Sample/target type | RNA template |
Mastermix | Yes |
Real-time or endpoint | End-point |
With/without hotstart | With hot-start |
Single or multiplex | Single/duplex |