QIAcube Connect

QIAGENサンプルテクノロジーを網羅した新しい自動核酸精製装置

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QIAcube Connect

Cat. No. / ID: 9002864

Instrument, 1-year warranty on parts and labor

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Service Options

Instrument

System PRV-1

System FUL-2

System FUL-3

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QIAcube Connectは、分子生物学実験で核酸やタンパク質の完全自動精製を行うために設計されています。本システムは、分子生物学的な技術およびQIAcubeConnect の操作研修を受けた専門ユーザーによる使用を目的としています。

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Features

3000以上のプロトコールで80以上のQIAGENキットを自動化
DNA、RNA、タンパク質サンプル処理を自動化
手作業による処理手順を排除
標準化された結果を提供

Product Details

QIAcube Connectは、広く使用されているQIAcubeの次世代モデルで、サンプル処理の自動化の新しい“かたち”です。QIAcubeで培った経験と実績をもとに、QIAcube Connectはボタンを押すだけで、広く研究室で利用されているQIAGENスピンカラムによる核酸抽出・精製操作を完全自動化で再現します。

QIAcube Connectは、新次元の実用性を提供するユーザーインターフェースを搭載しています。研究者は内蔵タッチスクリーンにより機器にアクセスすることができます。そのためQIAcube Connectから離れている間も素早い応答時間とランをモニターすることができます。

QIAcube Connectの詳細はQIAGENのデモツールをご参照ください

QIAcube Connectは専用の消耗品QIAcube Connect Accessoriesとともにご使用ください。

また、従来のQIAcube用ユーザーマニュアルやプロトコール等は QIAcube (classic) resource page からもダウンロードが可能です。

Performance

QIAcube Connectの自動サンプル調製技術は、いずれの研究室でも利用できる技術です。装置は信頼と実績のQIAGENスピンカラムキットを完全自動化し、精製作業の簡素化および合理化をします。試薬を変える必要はなく、迅速なセットアップとマニュアル操作を再現する期待通りの結果をお約束します。またQIAcube Connectを利用すれば、作業に費やす時間も短縮されるだけでなく、自動化による作業手順の標準化と生産性を向上させることができます。

Principle

QIAcube Connectは定評のあるQIAGENスピンカラムキットをそのまま自動化でき、面倒な手作業から解放されます。QIAcube Connectは、遠心分離機、加熱振とう機、ピペッティングシステム、およびロボットグリッパー（図 “QIAcube Connectワークテーブル”）などを総合的に制御管理できます。そのため、80以上のQIAGENスピンカラムキットを完全自動化することができます。

UVライトによる除染

QIAcube Connectの新しい内蔵UVライトで、ワークテーブルを簡単かつ効率的に完全に除染することができます(図 “QIAcube Connectの原理”）)。この機能によって、サンプル間のキャリーオーバーも防ぐのみならず、1台の機器で様々なサンプルタイプからDNA、RNA、病原体の核酸とタンパク質を精製することができます（図 “UVライト照射後ほとんど検出されないゲノムDNA (gDNA)”）。

リモート接続

新しいQIAcube Connectを使用すると、サンプル前処理の準備から結果を得るまでの時間が短縮され、研究について考える自由な時間が生まれます。 QIAcube Connectにはバーコードスキャナーが付属しています。サンプル調製キットのバーコードをスキャンするだけで、必要なすべてのキット情報がすぐに画面に表示されます。

照明付きのワークテーブル

QIAcube Connectの機器内部にはライトが付いているので、ワークテーブル上での作業効率が向上します。

様々な種類のサンプル用プロトコール

QIAcube Connectは、RNA、ゲノムDNA、プラスミドDNA、ウイルス核酸およびタンパク質の精製、さらにDNAおよびRNAのクリーンアップのためのさまざまなプロトコールを保持しています。さらにすべての拡張標準プロトコールも無料でダウンロードできます。特定のアプリケーションの要求に合わせてカスタマイズされたプロトコールも作成が可能です。

Procedure

QIAcube Connectは、は、溶解、結合、洗浄および溶出手順で、高純度の核酸またはタンパク質の精製が可能です（図 “QIAprep Mini のスタンダードな自動化プロセス”）。

革新的な使いやすさ

QIAcube Connectの使いやすさはこれまでのモデルと比べ物になりません。大型内蔵タッチスクリーン（図 “QIAcube Connectタッチスクリーン”）はプロトコールの選択を単純化し、画面上のメッセージにしたがってワークテーブルの設定をすることができます。使いやすさの向上と高いプロセス安全性のために、実験器具と付属品は正しい向きでワークテーブルにセットされるように設計されています。さらに、自動化されたロードチェックで、必要な試薬消耗品が確実にセットされているかを確認することができます。

DNAとRNA精製用キットを用意

QIAcube Connect用の核酸精製キットは、スピンカラムによる精製操作をさらに簡便化することができます。キットは現在、RNA、ゲノムDNAおよびウイルスRNA精製用のご用意があります。キットに同梱されているローターアダプターにはスピンカラムと溶出チューブがあらかじめセットされているので、あとは装置にセットするだけです。これらキットはQIAcube Connectでの使用を想定しているので、操作性は向上し、さらに、人為的エラーも低減できて生産性が向上します。またマニュアル操作では必要なチューブは同梱されないために経済的です。

Applications

QIAcube Connectは、以下のようなアプリケーションを実施する大学研究室や製薬企業、バイオテクノロジー企業に最適です。

シークエンシング/シークエンシング解析
遺伝子発現解析
ジェノタイピング
プロテオミクス解析
マイクロバイオーム研究

Services

研究室での核酸精製の検体数は増えていますか？　今後は多様なアプリケーションへの拡張やワークフローの効率化を検討していますでしょうか？　また、管理の複雑なサンプルを取り扱いますか？　作業を電子化して管理する必要がありますか？

もし、これらのうち一つでもあてはまることがあれば、QIAcube Connect をご検討ください。

QIAGENは、最新の自動化および核酸精製技術を駆使して、みなさまの研究を加速させます。

Supporting data and figures

The QIAcube Connect UV light

The QIAcube Connect instrument comes with a built-in UV light source that enables full worktable decontamination, and prevents any sample carryover.

Genomic DNA (gDNA) barely detectable after UV light exposure

Automated QIAprep Mini standard procedure

Specifications

Features	Specifications
Technical data	Power: 100–240 V AC, 50/60 Hz, 650 VA Mains supply voltage fluctuations are not to exceed 10% of nominal supply voltages. Note: The apparent power can exceed 650 VA for up to 2 seconds during the centrifuge acceleration and can reach an approximate value of 1000 VA.; Fuse: 2x T6.3A L 250V; Overvoltage category: II
Operating temperature	18 to 28ºC (64.4 to 82.4ºF)
Relative humidity	15–75% (non-condensing)
Place of operation	For indoor use only
Pollution level	2
Environmental class	3K2 (IEC 60721-3-3)
Storage conditions	Temperature: 15ºC to 30ºC (59ºF to 86ºF) in manufacturer’s package; Relative humidity: Max. 75% (non-condensing); Environmental class: 1K2 (IEC 60721-3-1)
Instrument dimensions	Dimensions (hood closed): width 65 cm (25.6 in.), height 58 cm (22.8 in.), depth 62 cm (24.4 in.); Dimensions (hood open): width 65 cm (25.6 in.), height 86 cm (34 in.), depth 62 cm (24.4 in.); Mass: QIAcube Connect: 71.5 kg (157.6 lb.), Accessories: 3 kg (6.6 lb.)
Centrifuge	10,640 rpm maximum, 12,000 x g maximum, swing-out rotor maximum 45°, 12 rotor positions
Shaker	Speed 100–2000 rpm, amplitude 2 mm, heating range of ambient temperature to 70°C (158°F), ramp-up time of <5 minutes from ambient temperature to 55°C (±3°C). Difference in the temperature detected by the internal sensor and the temperature of the sample liquid is approximately –2°C.
Pipetting system	Syringe size 1 ml, pipetting range 5–900 µl
Table capacity	Up to 12 samples per run
Touchscreen	10.4” TFT Touchscreen, active area 211.2 x 158.4 mm, resolution 800*600 SVGA
Data output	USB stick: USB2.0; Compatible OS: Windows 8, Windows 7, Windows Vista, Windows XP (SP3 or later); Mac OS x 10,1 or later; Operating temperature range: 0ºC to 35ºC; Operating humidity range: 10 to 90% (with no condensation); Storage temperature range: –20ºC to 60ºC (–4ºF to 140ºF); Storage humidity range: 10 to 90% (with no condensation); Formatting: FAT32
UV lamp	Wavelength: 278nm; Optical power: 200-300mW
Special feature	Handheld scanner; Scan pattern: Area Image (838 x 640-pixel array); Motion tolerance: Up to 610 cm/s (240 in/s) for 13 mil UPC at optimal focus; Symbol contrast: 20% minimum reflectance difference; Decode capability: Reads standard 1D, PDF, 2D, Postal and OCR symbology
Software	QIAGEN protocols are pre-installed on the QIAcube Connect.
Altitude	Up to 2000 m (6500 ft)
Transportation conditions	–25°C to 60°C (–13°F to 140°F) in manufacturer's package; Max. 75% relative humidity (non-condensing); Environmenal class: 2K2 (IEC 60721-3-2)

Service Plans

QIAcube Connect, Premium Agreement

Cat. No. / ID: 9245209

On-site QIAcube Connect instrument repair, including travel, labor and parts, for a period of 1 year. Response time of next business day. Includes 1 Preventive Maintenance or Inspection Service during the Premium Agreement period.

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QIAcube Connect, Core Agreement

Cat. No. / ID: 9245260

For the QIAcube Connect, one on-site repair service and one on-site Preventive Maintenance or Inspection Service, including travel, labor and spare parts for a period of one year. Response time of five business days. Includes a 10% discount on additional repair service during the Core Agreement period.

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QIAcube Connect, Preventive Subscription

Cat. No. / ID: 9245210

One on-site Preventive Maintenance or Inspection Service visit for the QIAcube Connect, including travel, labor and parts. Includes a 10% discount on repair services during the Preventive Subscription period.

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QIAcube Connect, Full Agreement

Cat. No. / ID: 9245208

On-site QIAcube Connect instrument repair, including travel, labor and parts, for a period of one year. Response time of two business days.

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Resources

A versatile workflow for the detection of low-abundance microbes

For use with QIAsphere Base

This protocol has been adapted to allow the user to replace the epithelial transfer and sperm pellet wash steps with an automated protocol using the QIAcube Connect.

The QIAcube Connect System and 21 CFR Part 11 Regulations

A versatile workflow for the detection of low-abundance microbes

For use with QIAsphere Base

The QIAcube Connect System and 21 CFR Part 11 Regulations

FAQ

For an up-to-date list of available protocols, visit QIAcube Standard Protocols.

Click here for a virtual demonstration on how the QIAcube operates.

FAQ ID -1403

Yes. The heated shaker on the QIAcube can be operated independently if the QIAcube is not performing a protocol run. For instructions on how to operate the shaker, refer to the QIAcube User Manual, chapter 5 'Operating Procedures, section 5.7 'Operating the shaker'.

The shaker can be used to heat and shake samples simultaneously, shake samples without heating, or heat samples without shaking.

See: http://www.qiagen.com/products/catalogpagecontrols/header/qiacube/360/default.aspx for a virtual demonstration of the heater and shaker on the QIAcube.

FAQ ID -1422

Visit the QIAcube Protocols page to search for protocols. The instructions for transferring a protocol to the QIAcube are listed below.

To transfer new protocols to the QIAcube, first save them to the supplied USB stick.

Select the protocol to be downloaded and save it to the “New_Protocols” folder on the USB stick.
Make sure that the QIAcube is switched on.
To install the protocol, connect the USB stick to the QIAcube via the USB port, which is located at the front right of the instrument.
In the main menu, press “Tools”.
Select “Data Exchange” by pressing the "Up Symbol" or the "Down Symbol" to scroll through the list until it is highlighted and then press “Select”.
To transfer protocols to the QIAcube, select “Protocols” and “Load from USB”.
Start the download procedure by pressing “OK”. A message will confirm that the protocols have been successfully transferred to the QIAcube.

Note: Protocols already installed on the QIAcube that have the same name as downloaded protocols will be overwritten.

8. When the protocols have been successfully transferred to the QIAcube, press “OK”.

FAQ ID -1421

Yes. At the end of each protocol run on the QIAcube, a report file is generated that contains important information such as number of samples, liquid volumes measured during the load check, and run time. A log file is also generated that contains information about instrument actions, such as movement of the robotic arm.

FAQ ID -1412

Yes. The QIAcube centrifuge can be operated independently if the QIAcube is not performing a protocol run. For instructions on how to operate the centrifuge, refer to the QIAcube User Manual, chapter 5 'Operating Procedures, section 5.8 'Operating the Centrifuge'.

FAQ ID -1423

Yes, this is absolutely necessary. If the lids are not securely fixed in the rotor adapter slots during processing on the QIAcube, they will become detached from the elution tubes and columns during centrifugation and will collect at the bottom of the centrifuge chamber.

FAQ ID -1415

The following disinfectants and detergents are recommended for cleaning the QIAcube:

Mikrozid® Liquid (Schülke & Mayr GmbH) — ethanol-based disinfectant for spraying onto surfaces (consists of 25 g ethanol and 35 g 1-propanol per 100 g Mikrozid Liquid)
RBS®-35 Detergent Concentrate (Pierce Chemical Co.) — anionic and nonionic surfactant based detergent for cleaning the touch screen and worktable door
RNaseZap® (Ambion) — for complete removal of RNase contamination from glass and plastic surfaces
0.1 M NaOH — for cleaning the worktable and racks to remove RNase contamination (alternative to RNaseZap)
Note: Alcohol is not recommended for cleaning of the door.

Europe

Lysetol® AF (Gigsept® Instru AF) (Schülke & Mayr GmbH) — disinfectant for submerging worktable items (consists of 14 g cocospropylene-diamine-guanidine diacetate, 35 g phenoxypropanols, and 2.5 g benzalkonium chloride per 100 g Lysetol AF, with anticorrosion components, fragrance, and 15–30% nonionic surfactants).

USA

DECON-QUAT® 100 (Veltek Associates, Inc.) — quaternary ammonium salt based disinfectant concentrate for submerging worktable items (contains 5% alkyldimethylbenzylammonium chloride and 5% alkyldimethylethylbenzylammonium chloride).

Note: If you want to use disinfectants different from those recommended, ensure that their compositions are similar to those described above.

FAQ ID -1416

New software or software updates can be accessed via the QIAcube Web Portal at www.qiagen.com/MyQIAcube. To access them directly, go to User Support.

FAQ ID -1413

No. The QIAcube is designed for fully automated processing of up to 12 samples using QIAGEN spin-column kits. Do not use spin columns manufactured by other suppliers with the QIAcube. Damage caused by use of other types of spin columns will void the instrument warranty.

See: http://www.qiagen.com/products/catalogpagecontrols/header/qiacube/360/default.aspx for a demonstration on how the QIAcube is used with the RNeasy Mini Kit for automated purification of RNA.

FAQ ID -1406

Protocols for automated DNA extraction from saliva stabilized with the PAXgene Saliva Collector are available for the QIAsymphony DNA Midi Kit with the QIAsymphony SP instrument or the QIAamp DNA Mini Kit with the QIAcube (Classic and Connect).
For manual extraction, supplementary protocols are available for the QIAamp DNA Mini and Gentra Puregene Cell Kits (see resources section).

3821

No. Modified QIAGEN protocols or custom protocols developed to meet your specific requirements can be requested and purchased from QIAGEN.

1. Go to www.qiagen.com/MyQIAcube and enter the required information. If our Application Specialists require additional information, they will contact you directly.

2. You will be informed by e-mail when your customized protocol is ready for download.

3. Click the link in the e-mail to go to the download page.

FAQ ID -1405

No, it is not possible to connect the QIAcube with laboratory computer networks.

FAQ ID -1411

Dedicated QIAcube kits are the RNeasy Mini QIAcube kit, QIAamp DNA Mini QIAcube Kit , the QIAamp DNA Blood Mini QIAcube Kit, and the QIAamp Viral RNA Mini QIAcube Kit.

See: http://www.qiagen.com/Products/Catalog/Automated-Solutions/Sample-Prep/QIAcube#orderinginformation for a virtual demonstration on how to use the QIAcube.

FAQ ID -2337

The QIAcube has the following weight and dimensions:

Weight: 70.5 kg (155.4 lb.)
Width: 65 cm (25.6 in.)
Depth: 62 cm (24.4 in.)
Height (door closed): 57 cm (22.4 in.)
Height (door open): 81 cm (32 in.)

FAQ ID -1407

Please refer to section 6.5 'Decontaminating the QIAcube' in chapter 6 'Maintenance Procedures' of the QIAcube User Manual.

FAQ ID -1419

Yes, rotor and/or buckets can be removed from the QIAcube for cleaning. For information on how to remove these parts, refer to the QIAcube User Manual.

FAQ ID -1410

You can stop a QIAcube protocol if there is an emergency by pressing “Cancel”. To confirm that you want to stop the protocol run, press “OK”. To cancel the 'stop protocol' command, press “Cancel”.

Note: If a protocol run is stopped, the run cannot be restarted. The samples must be processed manually. The last protocol step performed by the QIAcube will be displayed in the touch screen. For more information, refer to the QIAcube User Manual.

FAQ ID -1414

The corresponding manual kits can be used for all available protocols on the QIAcube. The manual kits contain sufficient buffers and reagents to process the number of spin columns provided with the kit.

In addition to the purification kit, Rotor Adapters (10 x 24), cat. no 990394, and the required disposable tips:

Filter-Tips, 200 µl (1024), cat no. 990332,
Filter-Tips, 1000 µl (1024), cat no. 990352,
Filter-Tips, 1000 µl wide-bore (1024), cat no. 990452,

need to be ordered separately. To find out which filter-tips are required for a specific application, refer to the corresponding protocol sheet.

FAQ ID -1402

No. Autoclaving the rotor adapters will cause the plastic to deform and the rotor adapters will no longer fit properly into the centrifuge buckets.

FAQ ID -1409

No. The QIAcube is equipped with an optical sensor, which determines the tip size and the number of tips loaded onto the worktable prior to starting a run.

The QIAcube can use partially used tip racks from previous runs, provided that the racks contain sufficient tips to process the number of samples loaded for the current run.

FAQ ID -1418