Isolation of bacteriophage-P1-derived constructs using the QIAGEN® Plasmid Midi Kit - (EN)

The procedure has been used successfully for isolation of 110 kb P1 DNA (pAdsacBII with an 80 kb insert) from Escherichia coli strain NS3529. Yield of P1 DNA was typically 10-50 µg from 500 ml culture.