QIAGEN Genomic-tips

For isolation of up to 20 µg, 100 µg or 500 µg high-molecular-weight DNA from a wide range of samples

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QIAGEN Genomic-tip 20/G

Cat. No. / ID: 10223

25 columns

JP¥24,500

Column type

20/G

100/G

500/G

QIAGEN Genomic-tips are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

Minimal mechanical shearing allows isolation of high-molecular-weight DNA up to 150 kb in size
No phenol or chloroform extractions
Convenient, parallel processing of multiple samples

Product Details

QIAGEN Genomic-tips are gravity-flow, anion-exchange tips that allow efficient purification of genomic DNA from a wide range of biological samples. The purified DNA is sized up to 150 kb with an average size of 50–100 kb

Performance

The QIAGEN Genomic-tip procedure is very gentle and results in negligible DNA shearing. DNA purified with QIAGEN Genomic-tips is sized up to 150 kb with an average length of 50–100 kb (see figure " Genomic DNA of up to 150 kb"). The DNA is free of all contaminants such as RNA, protein and metabolites and has A₂₆₀ / A₂₈₀ ratios between 1.7 and 1.9.

QIAGEN Genomic-tips come in a range of sizes to suit every preparation need and are supplied with a comprehensive handbook containing detailed protocols for genomic DNA preparation from each sample type. Specific lysis buffers for blood, tissue, cultured cells, yeast and bacteria and all other necessary buffers are provided in the Genomic DNA Buffer Set which can be purchased separately.

Purified DNA has been used in direct bacterial-genome sequencing (see figure " Direct sequencing of bacterial genomic DNA").

QIAGEN Genomic-tip specifications

Sample source	Amount			Yield (µg)
	Genomic-tip 20/G	Genomic-tip 100/G	Genomic-tip 500/G	Genomic-tip 20/G	Genomic-tip 100/G	Genomic-tip 500/G
Whole blood (human)	1 ml	5 ml	20 ml	15–20	80–100	350–400
Cultured cells (HeLa)	5 x 10⁶	2 x 10⁷	1 x 10⁸	15–20	80–100	350–450
Tissue (liver)	15 mg	80 mg	350 mg	15–20	80–100	350–450
Yeast (S. cerevisiae)	1 ml	5 ml	20 ml	18–20	85–95	350–450
Gram– bacteria (E. coli)	0.6–1.2 ml	3–6 ml	15–30 ml	16–20	85–95	300–400
Gram+ bacteria (B. subtilis)	1.2–1.8 ml	6–9 ml	30–45 ml	16–20	85–95	300–400

See figures

Genomic DNA of up to 150 kb.

Direct sequencing of bacterial genomic DNA.

Principle

QIAGEN Genomic-tips use unique QIAGEN anion-exchange technology for high-molecular-weight DNA isolation from a wide range of biological samples without phenol or chloroform. Lysis buffers are optimized for different sample types and provide immediate denaturation of proteins such as nucleases, histones and DNA-binding proteins, as well as potentially infectious viral particles. Under the pH and low-salt conditions provided by the buffer, DNA binds to the QIAGEN Resin in the column. At the same time, other cell constituents such as proteins, carbohydrates and metabolites flow through. Purified DNA is eluted in high-salt buffer. Genomic-tips operate by gravity flow, and can be left unattended without running dry. This reduces hands-on time to a minimum and makes the procedure ideal for simultaneous processing of multiple samples.

Procedure

Samples are first lysed (tissue samples are mechanically disrupted) and proteins simultaneously denatured in the appropriate lysis buffer (see flowchart " QIAGEN Genomic-tip procedure"). QIAGEN Protease or Proteinase K is then added and after a suitable incubation period, lysates are loaded onto the QIAGEN Genomic-tip. DNA binds to the column while other cell constituents pass through. Following a wash step to remove any remaining contaminants, pure, high-molecular-weight DNA is eluted and precipitated with isopropanol. Hands-on time for the complete procedure is just 20 minutes for bacteria, 30 minutes for blood and cultured cells and 40 minutes for tissue and yeast.

See figures

QIAGEN Genomic-tip procedure.

Applications

DNA purified with QIAGEN Genomic-tips is well suited for use in the following applications:

RFLP analysis
Analysis of gene targeting
Screening of transgenic animals
DNA fingerprinting studies
Southern blotting

Supporting data and figures

Exemplary size distribution using QIAGEN Genomic-tips (Cells)

Exemplary size distribution of DNA from cells using QIAGEN Genomic-tips. Samples were analyzed with the Femto Pulse System and signals were normalized to maximum peak.

Resources

The expected yield of genomic DNA from 0.5–2.5 ml of clotted whole blood should be in the range of 8–40 μg.

Lysis time will vary depending on the size and density of the source material. The QIAGEN Genomic-tip will run slowly due to the high debris content in the nuclear fraction. The yield is about 0.3-0.4 µg DNA/fly.

This protocol is designed for isolation of up to 200 μg RNA from 150 mg plant tissue or up to 1 mg RNA from 600 mg plant tissue and is for use with QIAGEN-tip 100 or QIAGEN-tip 500, respectively.

Download Safety Data Sheets for QIAGEN product components.

FAQ

Yes, please follow the User-Developed Protocol 'Isolation of genomic DNA from plants and filamentous fungi using the QIAGEN Genomic-tip' (QG08).

FAQ ID -905

Yes, please follow the User-Developed Protocol 'Isolation of genomic DNA from flies using the QIAGEN Genomic-tip 100/G' (QG05).

FAQ ID -903

Yes, we have the following protocols:

Isolation of genomic DNA from mosquitoes or other insects using the QIAGEN Genomic tip (QG06).
Purification of total DNA from insects using the DNeasy Blood & Tissue Kit (DY14).

FAQ ID -904

Using Gentra Puregene Cell kit:

• Purification of archive-quality DNA from up to 30 Drosophila melanogaster using the Gentra Puregene Cell Kit (PG20)

• Purification of archive-quality DNA from 100 Drosophila melanogaster using the Gentra Puregene Cell Kit (PG21)

• Purification of archive-quality DNA from 300–700 Drosophila melanogaster using the Gentra Puregene Cell Kit (PG22)

Using the QIAGEN Genomic tips:

• Isolation of genomic DNA from flies using the QIAGEN Genomic-tip 100/G (QG05)

FAQ ID -1970

Yes, please follow the User-Developed Protocol 'Isolation of genomic and plasmid DNA from cell cultures using the QIAGEN Genomic-tip' (QG04).

FAQ ID -902

Yes, we have the following protocols:

Isolation of genomic DNA from frozen clotted whole blood using the QIAGEN Genomic-tip 100/G (QG02). TEST

You will need to prepare the required buffers according to the recipes in Appendix A of the QIAGEN Genomic DNA Handbook, or you can purchase the Genomic DNA Buffer Set containing pre-made solutions. Alternatively, the QIAGEN Blood & Cell Culture Midi Kit, containing Genomic-tips 100/G and buffers, can be used.

Purification of archive-quality DNA from clotted whole blood using Clotspin Baskets and the Gentra Puregene Blood Kit (PG03).
Purification of archive-quality DNA from clotted whole blood using the Gentra Puregene Tissue Kit or Gentra Puregene Mouse Tail Kit (PG04).
Purification of DNA from clotted blood using the FlexiGene DNA Kit (FG01).

FAQ ID -900. Test

Yes, we have the following protocols:

Isolation of genomic DNA from fungi (culture and blood) using the QIAamp DNA Mini Kit (QA09).
Isolation of genomic DNA from plants and filamentous fungi using the QIAGEN Genomic-tip (QG08).

FAQ ID -911

Yes, please follow the Supplementary Protocols:

FAQ ID -907

Yes, please follow the User-Developed Protocol 'Isolation of genomic and viral DNA from lymphocytes using the QIAGEN Genomic-tip' (QG03). The protocol is for use with the QIAGEN Genomic-tip 20/G.

FAQ ID -901

Using QIAGEN Genomic-tips

Protocol for DNA cleanup using Genomic-tips can be found in the 'Special Applications' section of the QIAGEN Genomic DNA Handbook.

Using QIAamp DNA Micro kit

Up to 10ug of gDNA can be cleaned using the cleanup protocol in the QIAamp DNA Micro Handbook.

Using Gentra Puregene Reagents

Gentra Puregene Handbook has protocols for removal of proteins and RNA from purified gDNA in Appendix C and Appendix D respectively.

FAQ ID -618

Genomic DNA purified using QIAGEN Genomic-tips ranges in size from 20–150 kb, with an average length of 50–100 kb. Vortexing the lysate for about 20 seconds may reduce the size of the genomic DNA slightly to 20–130 kb, but can help to improve flow rates.

FAQ ID -142