Why is the fluorescence signal of the Internal Control on my Rotor-Gene Q very low?

Investigator Quantiplex Kits are ready-to-use quantitative real-time PCR assays for human identity testing and forensic applications with conventional 2 µL or flexible 1-18 µL sample input (Investigator Quantiplex Pro FLX Kit). Developed for superior sensitivity and reaction speed, they are designed to establish whether a sample contains sufficient and intact human DNA to enable STR or SNP analysis by Capillary Electrophoresis or NGS. An internal amplification control (IC) is used to test successful amplification and identify the presence of PCR inhibitors. The Investigator Quantiplex Pro technology further enables quality assessment to provide reliable information on the degradation status of your sample. Investigator Quantiplex Kits meet ISO 18385 requirements.