Frequently Asked Questions - FAQ



FAQ Bookmark シェア more.. You are not authorized to download the resource Sort options アルファベット順関連順 What is the composition of Buffer RLC? FAQ ID -2795 表示 What is the composition of Buffer RLT? FAQ ID -2793 表示 What is the composition of Buffer RLT plus? FAQ ID -2794 表示 What is the composition of Buffer BB? FAQ ID -2790 表示 What is the composition of Buffer ETR? FAQ ID -2789 表示 What is the composition of Buffer PB? FAQ ID -2791 表示 What are the key parameters contributing to unwanted off-target effects in an RNAi experiment? FAQ ID -2775 表示 What are the structures of the siRNA molecules used in RNAi studies? FAQ ID -2760 表示 What are the critical factors in designing the siRNA molecules to be used for RNAi studies? FAQ ID -2759 表示 Why is my EpiTect Methyl qPCR Assay "failed" as indicated in the QC page of the data analysis Excel file? FAQ ID -2734 表示 Why is the qRT-PCR reproducibility so critical when detecting gene expression knock down in an RNAi experiment? FAQ ID -2774 表示 What is the composition of gDNA Eliminator Solution? FAQ ID -2799 表示 What is the composition of Buffer RW1? FAQ ID -2796 表示 What is the minimum amount of genomic DNA required for analysis using EpiTect Methyl qPCR Arrays? FAQ ID -2739 表示 What is the composition of Buffer RWT? FAQ ID -2798 表示 Can miScript miRNA qPCR Assays be used for pre-miRNA detection? FAQ ID -2724 表示 Can miScript miRNA qPCR Assays detect single nucleotide difference between miRNAs? FAQ ID -2723 表示 Do I need an internal methylated DNA reference in the EpiTect Methyl qPCR Array? FAQ ID -2736 表示 Do I need the sequence specific miRNA primers in the RT step of the miRNA qPCR Assays? FAQ ID -2725 表示 Do you always run samples in triplicates? FAQ ID -2703 表示 Can you use stained (H & E) sections with RT² PreAMP cDNA Synthesis Kit? FAQ ID -2722 表示 Does QIAGEN guarantee the performance of the SureSilencing shRNA Plasmids? FAQ ID -2787 表示 Does this method with the EpiTect Methyl qPCR Array detect methylation at specific CpG dinucleotides? FAQ ID -2737 表示 Can I make stable cell lines with the Cignal Reporter Assays? FAQ ID -2763 表示 Is it acceptable to correct for transfection efficiency, when determining the level of gene expression knock down by an shRNA expression vector? FAQ ID -2783 表示 How are the primers in the EpiTect Methyl qPCR Array designed? FAQ ID -2735 表示 On which instrumentation will the RT² Profiler PCR Array work? FAQ ID -2719 表示 Should I use monoclonal or polyclonal antibodies in EpiTect ChIP assays? FAQ ID -2747 表示 What are the advantages of using the SureSilencing shRNA Plasmids over siRNA? FAQ ID -2786 表示 The EpiTect ChIP qPCR primers I used show very high Ct. Are there any solutions? FAQ ID -2750 表示 The pathway reporter luciferase activity values are greater than the positive control, is there a problem? FAQ ID -2766 表示 The pathway reporter luciferase activity values are less than the negative control, is there a problem? FAQ ID -2767 表示 How can I prevent the evaporation of reaction volume from the wells EpiTect Methyl qPCR Arrays? FAQ ID -2742 表示 How can I prevent the evaporation of reaction volume from the wells in the miScript miRNA PCR array? FAQ ID -2729 表示 How can RNA interference be used as a life science research tool? FAQ ID -2756 表示 How can I be sure that the restriction enzyme digestion is complete in the EpiTect Methyl qPCR system? FAQ ID -2731 表示 How can I identify the binding sites of my transcription factor using ChIP? FAQ ID -2753 表示 How can I normalize my EpiTect ChIP results? FAQ ID -2751 表示 What is the recommended amount of input template for each RT² qPCR Primer Assay? FAQ ID -2716 表示 What is the RT² Profiler PCR Array? FAQ ID -2718 表示 Can the EpiTect Methyl qPCR System technology reliably characterize heterogenous tissue samples? FAQ ID -2732 表示 Can users insert custom transcriptional regulatory elements or promoters into the Cignal Reporter Assays? FAQ ID -2765 表示 Can you custom design the Epitect qPCR primers for the CpG island outside of the defined promoter region? FAQ ID -2744 表示 Can I manually set the threshold line? FAQ ID -2702 表示 Can I use your EpiTect One-Day kit with transcription factors? FAQ ID -2752 表示 Are EpiTect Methyl qPCR Arrays or Assays applicable to FFPE samples? FAQ ID -2733 表示 Can I obtain the sequence of the RT² qPCR Primer Assay that I purchased? FAQ ID -2709 表示 Are there any unique elements that need to be incorporated into an shRNA expression vector? FAQ ID -2772 表示 Can I use total RNA for the miRNA PCR Arrays or Assays? FAQ ID -2726 表示 At what point can I stop and freeze my samples when using ChIP? FAQ ID -2754 表示 Can I use the default PCR program to run EpiTect Methyl qPCR Arrays or Assays? FAQ ID -2745 表示 Can I use your EpiTect ChIP system in a RNA study? FAQ ID -2748 表示 Can I transform the Cignal Reporter Assays? FAQ ID -2762 表示 Can I reliably detect intermediately methylated DNA with EpiTect Methyl qPCR Array technology? FAQ ID -2738 表示 How many experiments can I do with the Cignal Lenti Reporter Assays? FAQ ID -2769 表示 How many housekeeping genes are included in each PCR Array? FAQ ID -2704 表示 Can I use fluorescence microscopy to assess shRNA plasmid transfection efficiency in my model cell line of interest, when using a vector expressing a GFP reporter gene? FAQ ID -2782 表示 What are the advantages of EpiTect ChIP qPCR Assays and Arrays? FAQ ID -2721 表示 How reliable are the results from the miScript Primer Assays? FAQ ID -2730 表示 What are EpiTect ChIP qPCR Assays? FAQ ID -2720 表示 How do you determine the efficiency using the PCR array? FAQ ID -2701 表示 How do I choose EpiTect ChIP-grade antibodies? FAQ ID -2746 表示 How do you design your EpiTect ChIP qPCR Assay primers? Do they work with SB GR? FAQ ID -2749 表示 How does QIAGEN control the quality of the RT² qPCR Primer Assays? FAQ ID -2711 表示 How does stable transfection of a shRNA-encoding plasmid work? FAQ ID -2779 表示 How do I choose between the DNA-based Cignal Reporter Assays and the Cignal Lenti Reporter Assays? FAQ ID -2761 表示 What are the storage conditions for the Microbial DNA qPCR products? FAQ ID — 3394 表示 Which Microbial qPCR Mastermix should I use? FAQ ID — 3395 表示 What is the expected amplicon size of the Microbial DNA qPCR Assays? FAQ ID — 3396 表示 3326 - Which QIAcube standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet? 表示 3327 - How often should the o-ring for the pipettor tip-adapter be changed on the QIAcube? 表示 3328 - Can the Sample Tubes CB and the Sample Tubes RB be used interchangeably? 表示 3329 - When using REPLI-g, if the starting material is a single sorted cell, could mitochondrial DNA (mtDNA) be amplified? 表示 3330 - Is there any contamination from E. coli genomic DNA in the polymerase provided with the Repli-G Single Cell Kit? 表示 3331 - Should the REPLI-g amplified mtDNA be cleaned up before sequencing? 表示 3332 - Is there a method that can distinguish between non-amplified DNA and REPLI-g amplified DNA? 表示 3333 - When using REPLI-g, what is the purpose of the DTT added to the denaturation buffer in the cells/blood protocol? 表示 What sample types can be tested on the arrays/assays? FAQ ID — 3399 表示 What is the difference between Positive PCR Control (PPC) and Microbial DNA Positive Control? FAQ ID — 3397 表示 Can I use the Microbial DNA-Free Water and Microbial qPCR Mastermix if they have been opened more than 3 times? FAQ ID — 3398 表示 What are the minimum sample requirements for Microbial DNA qPCR kits? FAQ ID — 3393 表示表示 What is the QIAxcel QX RNA Size Marker 200-6000nt? FAQ ID - 3365 表示 What is the nature of the gel in the QIAxcel RNA QC kit v2.0? Is it denaturing? FAQ ID - 3366 表示 3352 - What are the size ranges of Ni-NTA particles? FAQ ID - 3352 表示 3353 - What is the composition of the elution buffer in the Ni-NTA Fast Start Kit? FAQ ID - 3353 表示 3351 - What is the upper limit for protein size that can bind to Ni-NTA agarose resin? FAQ ID - 3351 表示 3354 - What type and amount of resin is packed into the Ni-NTA Spin columns from the Ni-NTA Spin Kit? FAQ ID - 3354 表示 3341 - How are the EpiTect DNA controls produced and tested? FAQ - 3341 表示 3344 - What is the difference between the EpiTect Plus DNA Bisulfite kit compared to the classic kit? FAQ - 3344 表示 3345 - What is the difference between the high and low concentration EpiTect Bisulfite Conversion protocol? FAQ - 3345 表示 Why does AllPrep DNA/RNA/Protein Mini kit use buffer RLT, but Allprep DNA/RNA Mini and Allprep DNA/RNA Micro kits use buffer RLT Plus? Are these buffers interchangeable? FAQ ID - 3391 表示 3311 - Can QuickLyse miniprep kits be used with QIAVac 24 Plus? 表示 3312 - For DirectPrep 96 miniprep, is it possible to use a half plate and save the other half for later prep? 表示 3313 - What is the endotoxin level for the QIAGEN Plasmid Plus kits? 表示 3314-What is the concentration of RNase A sold separately? 表示 CAn I use milk or other liquids with the DNeasy mericon Food kit? What volume would I use? FAQ ID - 3350 表示 3316-What is the concentration of the reconstituted QuantiFast Probe Assay? 表示 Does salt concentration in the food matter when using the DNeasy mericon Food Kit? FAQ ID - 3346 表示 Why does the DNeasy mericon Food Kit use a QIAquick column and Buffer PB rather than a DNeasy column and Buffer AL, which might be expected since the kit isolates genomic DNA? FAQ ID - 3347 表示 When would I use the mericon DNA Bacteria Kit vs the mericon DNA Bacteria Plus Kit? FAQ ID - 3348 表示 Why is my 260/280 ratio low after using the DNeasy mericon Food Kit? FAQ ID - 3349 表示 Is it possible to stop the DNeasy tissue protocol and store the tissue lysates after digesting in buffer ATL and Proteinase K? FAQ ID - 3362 表示 What is the composition of QIAzol? What is the color of this reagent? FAQ ID - 3355 表示 Are QIAcube reagent bottles (cat. no. 990393) autoclavable? What type of plastic are they made of? FAQ ID - 3369 表示 Can I buy the gDNA eliminator plates supplied in your RNeasy Plus 96 kit separately? FAQ ID - 3389 表示 What is the composition of elution buffers used in QIAsymphony DNA Investigator kits? FAQ ID - 3387 表示 Can I buy the gDNA eliminator columns supplied in your RNeasy Plus kits separately? FAQ ID - 3390 表示 3323 - Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube? FAQ ID - 3323 表示表示 3340 - Which operating system is compatible with PyroMark IdentiFire Software? 表示 3325 - What are the catalog numbers for the packing materials for the QIAcube? 表示 3324 - Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube? 表示 3335 - Can I use the DNA directly from enzymatic reaction for Epitect Bisulfite treatment with the Epitect Bisulfite kit (cat. no. 59104)? 表示 3336 - Can I use the linearized DNA for Epitect Bisulfite treatment with the Epitect Bisulfite kit? 表示 3337 - Which method is used for QIAGEN sequencing service? 表示 3339 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) negatively impact subsequent NGS sequencing? 表示 3338 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) have 3'-hydroxyl and 5'-phosphate groups? 表示 3342 - How is the unmethylated unconverted EpiTect DNA control to be used? FAQ - 3342 表示 3343 - Why is it difficult to quantify DNA using UV/Vis spectroscopy and fluorescence methods following EpiTect Bisulfite Conversion? How best do you quantify it? FAQ - 3343 表示 3315-What is the concentration of the reconstituted QuantiTect Primer Assay (200)? 表示 3322 - What is the concentration of the reconstituted miScript Primer Assay (100) and miScript Precursor Assay (100) tubes? 表示 3317 - Do you have a protocol for isolation of genomic DNA from cell cultures using the QIAamp cador Pathogen Mini Kit? 表示 3318 - What guidelines should I follow in designing primers for use with the EpiTect MSP Kit? 表示 3320 - What is the sensitivity of the Type-it HRM PCR Kit? 表示 3318 - What is the preferred amplicon size for performing PCR on DNA amplified with the EpiTect Whole Bisulfitome Kit? 表示 3321 - What is the purpose of the C. elegans miR-39 spike-in control in the miRNeasy Serum/Plasma kit? 表示 Can I buy the RNeasy Mini columns, RNeasy MinElute columns, RNeasy Midi columns or the RNeasy Maxi columns separately? FAQ ID - 3388 表示 Do you have data to show lowest dropout rate with Repli-G WTA Single Cells kit? FAQ ID - 3392 表示 3305 - What is the minimal amount of starting material required for using the SBTexcellerator HLA Kits? 表示表示表示表示 3306 - What is the optimal sample DNA concentration for the Investigator ESSplex SE Plus Kit? 表示 3304 - What is the maximum DNA input volume that can be used in the Investigator ESSplex SE Plus Kit? 表示 3310 - Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator ESSplex SE Plus Kit? 表示 3308 - Where can I find the template files for the Investigator ESSplex SE Plus Kit? 表示 3309 - Which filter set should be used with Investigator ESSplex SE Plus Kit? 表示 What additional equipment is needed to purify RNA using the PAXgene 96 Blood RNA Kit? FAQ ID -499 表示 What fluorescent labels are 6-FAM, BTG, BTY, BTR, and BTO in Matrix Standard BT5 single or multi.cap? FAQ ID - 3364 表示 3300 - Can isopropanol or methanol be used in place of ethanol in RNeasy kits? 表示 3301 - Do you have any information or guidelines regarding the choice of reference genes for real-time PCR? 表示 I cannot analyze the data from your Investigator HID kits with my GeneMapper software. What can I do? FAQ ID - 3363 表示 3302 - How long can I store my purified RNA? 表示 Do you sell CoralLoad dye separately? FAQ ID - 3368 表示 3198 - What is the optimal DNA concentration for the Investigator DIPlex Kit? 表示 3194 - What DNA fragment sizes are required for the Investigator DIPlex kit? 表示 3195 - Where can I find the Population Data for the Investigator DIPlex kit? 表示 3196 - What is the DIPsorter Freeware? 表示 3188 - How many His residues are required for successful purification on Ni-NTA resins? 表示 3190 - What is the smallest elution volume for RNeasy Mini Spin column? 表示 Is it possible to extract RNA from RNAprotect-stabilized-blood? FAQ ID -3120 表示 What's the pulsed-field gel electrophoresis (PFGE) conditions of the gel picture showing on your website? http://www.qiagen.com/Search/MagAttract-HMW-DNA-Kit#productdetails -48 表示 Is it possible to extract RNA from frozen blood? FAQ ID -3119 表示 What is the composition of elution buffer ATE in the QIAamp DNA Investigator kit, QIAamp DNA FFPE Tissue kit and the QIAamp Fast DNA Stool Mini kit? FAQ ID -3122 表示 Why does the QIAcube sometimes fail to pick up the tips or pick up wrong tips? FAQ ID -3131 表示 I want to purify RNA from heart tissue. What is the best kit to use? FAQ ID -3116 表示 The kit says I can use up to 30mg of tissue. When I used 15mg I got a higher yield than when I used 30mg. Why? FAQ ID -3117 表示 Why does the upper aqueous phase look pinkish when purifying RNA from fatty tissue? FAQ ID -3118 表示 Is it possible to partially use RNeasy 96 plates and use the plate multiple times? FAQ ID -3124 表示 Can Dithiothreitol (DTT) be used instead of BME in Buffer RLT Plus of RNeasy Plus Mini Kit? FAQ ID -3125 表示 Can I use the QIAGEN deparaffinization solution with the IDH 1/2 RGQ PCR kit? FAQ ID -3100 表示 Can I use stained slide materials with IDH 1/2 RGQ PCR kit? FAQ ID -3101 表示 Do you have a protocol for obtaining > 20 µg plasmid DNA using the QIAprep spin miniprep kit? FAQ ID -3102 表示 How to upload a Flexiplate on QIAGEN Webpage? FAQ ID -3103 表示 How many transfections can be performed with Flexiplate siRNAs? FAQ ID -3105 表示 What is the standard format for Flexiplate siRNAs delivery? FAQ ID -3104 表示 Where to look for ordering Custom siRNAs online? FAQ ID -3106 表示 What is the difference between HiPerFect and HiPerFect HTS? FAQ ID -3108 表示 Can we do modification XXXXX for custom siRNA that is not on the web page? FAQ ID -3107 表示次ページ Back to top Contact QIAGEN Global contacts Technical Service Customer Care