Frequently Asked Questions - FAQ



FAQ Bookmark シェア more.. You are not authorized to download the resource Sort options アルファベット順関連順 The pathway reporter luciferase activity values are greater than the positive control, is there a problem? FAQ ID -2766 表示 The pathway reporter luciferase activity values are less than the negative control, is there a problem? FAQ ID -2767 表示 How can I prevent the evaporation of reaction volume from the wells EpiTect Methyl qPCR Arrays? FAQ ID -2742 表示 How can I prevent the evaporation of reaction volume from the wells in the miScript miRNA PCR array? FAQ ID -2729 表示 How can RNA interference be used as a life science research tool? FAQ ID -2756 表示 How can I be sure that the restriction enzyme digestion is complete in the EpiTect Methyl qPCR system? FAQ ID -2731 表示 How can I identify the binding sites of my transcription factor using ChIP? FAQ ID -2753 表示 How can I normalize my EpiTect ChIP results? FAQ ID -2751 表示 What is the recommended amount of input template for each RT² qPCR Primer Assay? FAQ ID -2716 表示 What is the RT² Profiler PCR Array? FAQ ID -2718 表示 Can the EpiTect Methyl qPCR System technology reliably characterize heterogenous tissue samples? FAQ ID -2732 表示 Can users insert custom transcriptional regulatory elements or promoters into the Cignal Reporter Assays? FAQ ID -2765 表示 Can you custom design the Epitect qPCR primers for the CpG island outside of the defined promoter region? FAQ ID -2744 表示 Can I manually set the threshold line? FAQ ID -2702 表示 Can I use your EpiTect One-Day kit with transcription factors? FAQ ID -2752 表示 Are EpiTect Methyl qPCR Arrays or Assays applicable to FFPE samples? FAQ ID -2733 表示 Can I obtain the sequence of the RT² qPCR Primer Assay that I purchased? FAQ ID -2709 表示 Are there any unique elements that need to be incorporated into an shRNA expression vector? FAQ ID -2772 表示 Can I use total RNA for the miRNA PCR Arrays or Assays? FAQ ID -2726 表示 At what point can I stop and freeze my samples when using ChIP? FAQ ID -2754 表示 Can I use the default PCR program to run EpiTect Methyl qPCR Arrays or Assays? FAQ ID -2745 表示 Can I use your EpiTect ChIP system in a RNA study? FAQ ID -2748 表示 Can I transform the Cignal Reporter Assays? FAQ ID -2762 表示 Can I reliably detect intermediately methylated DNA with EpiTect Methyl qPCR Array technology? FAQ ID -2738 表示 How many experiments can I do with the Cignal Lenti Reporter Assays? FAQ ID -2769 表示 How many housekeeping genes are included in each PCR Array? FAQ ID -2704 表示 Can I use fluorescence microscopy to assess shRNA plasmid transfection efficiency in my model cell line of interest, when using a vector expressing a GFP reporter gene? FAQ ID -2782 表示 What are the advantages of EpiTect ChIP qPCR Assays and Arrays? FAQ ID -2721 表示 How reliable are the results from the miScript Primer Assays? FAQ ID -2730 表示 What are EpiTect ChIP qPCR Assays? FAQ ID -2720 表示 How do you determine the efficiency using the PCR array? FAQ ID -2701 表示 How do I choose EpiTect ChIP-grade antibodies? FAQ ID -2746 表示 How do you design your EpiTect ChIP qPCR Assay primers? Do they work with SB GR? FAQ ID -2749 表示 How does QIAGEN control the quality of the RT² qPCR Primer Assays? FAQ ID -2711 表示 How does stable transfection of a shRNA-encoding plasmid work? FAQ ID -2779 表示 How do I choose between the DNA-based Cignal Reporter Assays and the Cignal Lenti Reporter Assays? FAQ ID -2761 表示 What are the storage conditions for the Microbial DNA qPCR products? FAQ ID — 3394 表示 Which Microbial qPCR Mastermix should I use? FAQ ID — 3395 表示 What is the expected amplicon size of the Microbial DNA qPCR Assays? FAQ ID — 3396 表示 3326 - Which QIAcube standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet? 表示 3327 - How often should the o-ring for the pipettor tip-adapter be changed on the QIAcube? 表示 3328 - Can the Sample Tubes CB and the Sample Tubes RB be used interchangeably? 表示 3329 - When using REPLI-g, if the starting material is a single sorted cell, could mitochondrial DNA (mtDNA) be amplified? 表示 3330 - Is there any contamination from E. coli genomic DNA in the polymerase provided with the Repli-G Single Cell Kit? 表示 3331 - Should the REPLI-g amplified mtDNA be cleaned up before sequencing? 表示 3332 - Is there a method that can distinguish between non-amplified DNA and REPLI-g amplified DNA? 表示 3333 - When using REPLI-g, what is the purpose of the DTT added to the denaturation buffer in the cells/blood protocol? 表示 What sample types can be tested on the arrays/assays? FAQ ID — 3399 表示 What is the difference between Positive PCR Control (PPC) and Microbial DNA Positive Control? FAQ ID — 3397 表示 Can I use the Microbial DNA-Free Water and Microbial qPCR Mastermix if they have been opened more than 3 times? FAQ ID — 3398 表示 What are the minimum sample requirements for Microbial DNA qPCR kits? FAQ ID — 3393 表示表示 What is the QIAxcel QX RNA Size Marker 200-6000nt? FAQ ID - 3365 表示 What is the nature of the gel in the QIAxcel RNA QC kit v2.0? Is it denaturing? FAQ ID - 3366 表示 3352 - What are the size ranges of Ni-NTA particles? FAQ ID - 3352 表示 3353 - What is the composition of the elution buffer in the Ni-NTA Fast Start Kit? FAQ ID - 3353 表示 3351 - What is the upper limit for protein size that can bind to Ni-NTA agarose resin? FAQ ID - 3351 表示 3354 - What type and amount of resin is packed into the Ni-NTA Spin columns from the Ni-NTA Spin Kit? FAQ ID - 3354 表示 3341 - How are the EpiTect DNA controls produced and tested? FAQ - 3341 表示 3344 - What is the difference between the EpiTect Plus DNA Bisulfite kit compared to the classic kit? FAQ - 3344 表示 3345 - What is the difference between the high and low concentration EpiTect Bisulfite Conversion protocol? FAQ - 3345 表示 Why does AllPrep DNA/RNA/Protein Mini kit use buffer RLT, but Allprep DNA/RNA Mini and Allprep DNA/RNA Micro kits use buffer RLT Plus? Are these buffers interchangeable? FAQ ID - 3391 表示 3311 - Can QuickLyse miniprep kits be used with QIAVac 24 Plus? 表示 3312 - For DirectPrep 96 miniprep, is it possible to use a half plate and save the other half for later prep? 表示 3313 - What is the endotoxin level for the QIAGEN Plasmid Plus kits? 表示 3314-What is the concentration of RNase A sold separately? 表示 CAn I use milk or other liquids with the DNeasy mericon Food kit? What volume would I use? FAQ ID - 3350 表示 3316-What is the concentration of the reconstituted QuantiFast Probe Assay? 表示 Does salt concentration in the food matter when using the DNeasy mericon Food Kit? FAQ ID - 3346 表示 Why does the DNeasy mericon Food Kit use a QIAquick column and Buffer PB rather than a DNeasy column and Buffer AL, which might be expected since the kit isolates genomic DNA? FAQ ID - 3347 表示 When would I use the mericon DNA Bacteria Kit vs the mericon DNA Bacteria Plus Kit? FAQ ID - 3348 表示 Why is my 260/280 ratio low after using the DNeasy mericon Food Kit? FAQ ID - 3349 表示 Is it possible to stop the DNeasy tissue protocol and store the tissue lysates after digesting in buffer ATL and Proteinase K? FAQ ID - 3362 表示 What is the composition of QIAzol? What is the color of this reagent? FAQ ID - 3355 表示 Are QIAcube reagent bottles (cat. no. 990393) autoclavable? What type of plastic are they made of? FAQ ID - 3369 表示 Can I buy the gDNA eliminator plates supplied in your RNeasy Plus 96 kit separately? FAQ ID - 3389 表示 What is the composition of elution buffers used in QIAsymphony DNA Investigator kits? FAQ ID - 3387 表示 Can I buy the gDNA eliminator columns supplied in your RNeasy Plus kits separately? FAQ ID - 3390 表示 3323 - Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube? FAQ ID - 3323 表示表示 3340 - Which operating system is compatible with PyroMark IdentiFire Software? 表示 3325 - What are the catalog numbers for the packing materials for the QIAcube? 表示 3324 - Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube? 表示 3335 - Can I use the DNA directly from enzymatic reaction for Epitect Bisulfite treatment with the Epitect Bisulfite kit (cat. no. 59104)? 表示 3336 - Can I use the linearized DNA for Epitect Bisulfite treatment with the Epitect Bisulfite kit? 表示 3337 - Which method is used for QIAGEN sequencing service? 表示 3339 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) negatively impact subsequent NGS sequencing? 表示 3338 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) have 3'-hydroxyl and 5'-phosphate groups? 表示 3342 - How is the unmethylated unconverted EpiTect DNA control to be used? FAQ - 3342 表示 3343 - Why is it difficult to quantify DNA using UV/Vis spectroscopy and fluorescence methods following EpiTect Bisulfite Conversion? How best do you quantify it? FAQ - 3343 表示 3315-What is the concentration of the reconstituted QuantiTect Primer Assay (200)? 表示 3322 - What is the concentration of the reconstituted miScript Primer Assay (100) and miScript Precursor Assay (100) tubes? 表示 3317 - Do you have a protocol for isolation of genomic DNA from cell cultures using the QIAamp cador Pathogen Mini Kit? 表示 3318 - What guidelines should I follow in designing primers for use with the EpiTect MSP Kit? 表示 3320 - What is the sensitivity of the Type-it HRM PCR Kit? 表示 3318 - What is the preferred amplicon size for performing PCR on DNA amplified with the EpiTect Whole Bisulfitome Kit? 表示 3321 - What is the purpose of the C. elegans miR-39 spike-in control in the miRNeasy Serum/Plasma kit? 表示 Can I buy the RNeasy Mini columns, RNeasy MinElute columns, RNeasy Midi columns or the RNeasy Maxi columns separately? FAQ ID - 3388 表示 Do you have data to show lowest dropout rate with Repli-G WTA Single Cells kit? FAQ ID - 3392 表示 3305 - What is the minimal amount of starting material required for using the SBTexcellerator HLA Kits? 表示表示表示表示 3306 - What is the optimal sample DNA concentration for the Investigator ESSplex SE Plus Kit? 表示 3304 - What is the maximum DNA input volume that can be used in the Investigator ESSplex SE Plus Kit? 表示 3310 - Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator ESSplex SE Plus Kit? 表示 3308 - Where can I find the template files for the Investigator ESSplex SE Plus Kit? 表示 3309 - Which filter set should be used with Investigator ESSplex SE Plus Kit? 表示 What additional equipment is needed to purify RNA using the PAXgene 96 Blood RNA Kit? FAQ ID -499 表示 What fluorescent labels are 6-FAM, BTG, BTY, BTR, and BTO in Matrix Standard BT5 single or multi.cap? FAQ ID - 3364 表示 3300 - Can isopropanol or methanol be used in place of ethanol in RNeasy kits? 表示 3301 - Do you have any information or guidelines regarding the choice of reference genes for real-time PCR? 表示 I cannot analyze the data from your Investigator HID kits with my GeneMapper software. What can I do? FAQ ID - 3363 表示 3302 - How long can I store my purified RNA? 表示 Do you sell CoralLoad dye separately? FAQ ID - 3368 表示 3198 - What is the optimal DNA concentration for the Investigator DIPlex Kit? 表示 3194 - What DNA fragment sizes are required for the Investigator DIPlex kit? 表示 3195 - Where can I find the Population Data for the Investigator DIPlex kit? 表示 3196 - What is the DIPsorter Freeware? 表示 3188 - How many His residues are required for successful purification on Ni-NTA resins? 表示 3190 - What is the smallest elution volume for RNeasy Mini Spin column? 表示 Is it possible to extract RNA from RNAprotect-stabilized-blood? FAQ ID -3120 表示 What's the pulsed-field gel electrophoresis (PFGE) conditions of the gel picture showing on your website? http://www.qiagen.com/Search/MagAttract-HMW-DNA-Kit#productdetails -48 表示 Is it possible to extract RNA from frozen blood? FAQ ID -3119 表示 What is the composition of elution buffer ATE in the QIAamp DNA Investigator kit, QIAamp DNA FFPE Tissue kit and the QIAamp Fast DNA Stool Mini kit? FAQ ID -3122 表示 Why does the QIAcube sometimes fail to pick up the tips or pick up wrong tips? FAQ ID -3131 表示 I want to purify RNA from heart tissue. What is the best kit to use? FAQ ID -3116 表示 The kit says I can use up to 30mg of tissue. When I used 15mg I got a higher yield than when I used 30mg. Why? FAQ ID -3117 表示 Why does the upper aqueous phase look pinkish when purifying RNA from fatty tissue? FAQ ID -3118 表示 Is it possible to partially use RNeasy 96 plates and use the plate multiple times? FAQ ID -3124 表示 Can Dithiothreitol (DTT) be used instead of BME in Buffer RLT Plus of RNeasy Plus Mini Kit? FAQ ID -3125 表示 Can I use the QIAGEN deparaffinization solution with the IDH 1/2 RGQ PCR kit? FAQ ID -3100 表示 Can I use stained slide materials with IDH 1/2 RGQ PCR kit? FAQ ID -3101 表示 Do you have a protocol for obtaining > 20 µg plasmid DNA using the QIAprep spin miniprep kit? FAQ ID -3102 表示 How to upload a Flexiplate on QIAGEN Webpage? FAQ ID -3103 表示 How many transfections can be performed with Flexiplate siRNAs? FAQ ID -3105 表示 What is the standard format for Flexiplate siRNAs delivery? FAQ ID -3104 表示 Where to look for ordering Custom siRNAs online? FAQ ID -3106 表示 What is the difference between HiPerFect and HiPerFect HTS? FAQ ID -3108 表示 Can we do modification XXXXX for custom siRNA that is not on the web page? FAQ ID -3107 表示 What is the stability of siRNA lyophilized and resuspended? FAQ ID -3109 表示 Why does my purified DNA/RNA sometimes have a 260/280 or 260/230 ratio of more than 2? Does that mean the purity is poor? FAQ ID -3132 表示 What are the miScript Controls that can be used with miScript HiSpec Buffer or miScript HiFlex Buffer? FAQ ID -3111 表示 Do you offer FlexiTube or FlexiPlate siRNAs for rat? FAQ ID -3114 表示 What is the amplicon size of the PCR product when using the miScript primer assay? FAQ ID -3156 表示 What are the stopping points in the QIAamp protocol? FAQ ID -3157 表示 What is the difference between EpiTect Bisulfite Kit and EpiTect Plus Bisulfite Kit? FAQ ID -3167 表示 What are the benefits of PyroMark Q24 Advanced versus PyroMark Q24? FAQ ID -3158 表示 Is it possible to use PyroMark Q24 Advanced reagents on PyroMark Q24 system? FAQ ID -3160 表示 Is it possible to run therascreen mutation or therascreen Pyro kits on PyroMark Q24 Advanced system? FAQ ID -3161 表示 Is it possible to upgrade existing PyroMark Q24 instruments to PyroMark Q24 Advanced or do I have to purchase a new instrument? FAQ ID -3162 表示 Do you have data on the DNA yield at different incubation time for the Repli-G Kits? FAQ ID -3113 表示 How do I perform the PyroMark Q24 Advanced upgrade? FAQ ID -3163 表示 Which operating system is compatible with PyroMark Q24 Advanced application software? FAQ ID -3164 表示 What are the differences between new PyroMark Q24 Advanced reagents chemistry compared to PyroMark Q24 Gold reagents? FAQ ID -3165 表示 What is the miRNA yield from the miRNeasy Serum/Plasma Kit? FAQ ID -3171 表示 What is the adapted annealing protocol for PyroMark Q24 Advanced procedure? FAQ ID -3166 表示 What is the RNA size distribution recovered from the miRNeasy Serum/Plasma Kit? FAQ ID -3172 表示 What is the difference between the 1 nmol FlexiTube and FlexiTube Gene Solution siRNAs? FAQ ID -3174 表示 What could be a reason for higher viscosity of PCR reaction with STR Plus chemistry? FAQ ID -3176 表示 Does QIAGEN provide template files for GeneMapper® 5 software? FAQ ID -3177 表示 Can the Rotor-Gene Q perform gradient PCR? FAQ ID -3183 表示 Can Rotor-Gene Reports be modified? FAQ ID -3184 表示 What is the best way to ensure the Rotor-Disc is properly sealed when using the Heat Sealer? FAQ ID -3179 表示 What is needed for performing delta delta Ct relative quantitation on the Rotor-Gene Q? FAQ ID -3178 表示 What to do if there is a loss of communication for the Rotor-Gene Q? FAQ ID -3180 表示 Aside from the OTV test, what other maintenance should be done for the Rotor-Gene Q? FAQ ID -3181 表示 3187 - Why does the Ni-NTA resin turn reddish-brown after mixing with the lysates? 表示 Are the miRNA Target Protector & miRNA mimic bench tested? Any guarantee? FAQ ID - 3186 表示 3191 - Is the Allprep DNA Mini Spin Column the same as the gDNA eliminator column? Can I interchange these columns? 表示 3192 - Is QIAGEN Protease compatible with Buffer ATL? 表示 3199 - Which species can be used with the GeneRead rRNA depletion kit? 表示 3193 - Where can I get an RNase-free syringe that is recommended for reconstituting QIAGEN's RNase-free DNase (cat. no. 79254)? 表示 What is the bacterial ribosomal RNA recommended to use in the miRNeasy Serum/Plasma Protocol? FAQ ID -3173 表示 Would co-transfection with the plasmid be expected to increase/decrease the transfection efficiency significantly? FAQ ID -3136 表示 How do I remove the RNA contamination from purified DNA? FAQ ID -3133 表示 How can I know whether there is any genomic DNA present in my purified plasmid? FAQ ID -3134 表示 Is it possible to use RNAprotect Cell Reagent to stabilize buffy coat? FAQ ID -3139 表示 How do I know whether my miRNA has been successfully purified and how much is expected? FAQ ID -3135 表示 What is the final concentration of Protease upon reconstitution in the QIAamp DNA Blood Mini? FAQ ID -3140 表示 Has QIAGEN ever tested Proteinase K treatment for a time period longer than overnight? 表示 Does RNAprotect affect morphology of tissues? FAQ ID -3142 表示 Is RNAprotect also suitable for the stabilization of miRNA? FAQ ID -3143 表示 What is the maximum cDNA length that can be synthesized by QuantiTect Reverse Transcription Kit? FAQ ID -3146 表示 Are miScript primer assays bench-validated? FAQ ID -3145 表示 What are the minimal and maximum fragment sizes for the QIAquick column in the mericon DNeasy kit? FAQ ID -3147 表示 Why does the DNeasy mericon Food extraction kit use a QIAquick column and not DNeasy column? FAQ ID -3148 表示 Can single-stranded DNA be removed by gDNA Eliminator columns? FAQ ID -3152 表示 How is total RNA purified with RNeasy kits free of genomic DNA contamination? FAQ ID -3154 表示 Is it possible to quantify protein content in RLT lysates? FAQ ID -3155 表示 Does RNase-free DNase I affect the isolation of dsRNA? FAQ ID -3153 表示 What primary cell lines have been tested with HiPerFect Transfection Reagent? FAQ ID -3137 表示 Is it possible to use the protein resuspended in ALO buffer for downstream BCA assay and Bradford assay? FAQ ID -3138 表示 What are the miScript Controls that can be used with miScript HiFlex Buffer ONLY? FAQ ID -3112 表示 How long can I store an RLT lysate? FAQ ID -3115 表示 What could be a reason for cloudy precipitate in PCR reaction with STR Plus chemistry after cycling protocol is completed? FAQ ID -3175 表示 Do you have a protocol for the isolation of genomic DNA from yeast/fungi directly from Blood? FAQ ID -3150 表示 Is REST software Windows 7 compatible? FAQ ID -3182 表示 Is there cross talk between channels in the Rotor-Gene Q? FAQ ID -3185 表示 Can QIAzol aqueous phase be used with the gDNA eliminator columns in RNeasy Plus mini kit? FAQ ID -3151 表示次ページ Back to top Contact QIAGEN Global contacts Technical Service Customer Care