| Cell Line Species/Tissue: |
Mouse
/
Embryonal Carcinoma |
| Transfection Reagent: |
HiPerFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
DMEM/ High Glucose |
Percent Serum (%):
|
10% |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
96-Well |
Number of Cells:
|
5,000 |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
75 nM |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
2 µl |
Complex Incubation on Cells (hrs):
|
48 hrs |
Analysis Performed Post-Transfection (hrs):
|
48 hrs |
Transfection Efficiency (%):
|
|
| Knockdown Efficiency (%): |
78% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Modification of Rervese Transfection Protocol: 1. Spotting of siRNA in 15 µl (medium without serum), 2. Addition of HiPerFect-dilution (15 µl per well; medium without serum), 3. incubation for 10 minutes at RT, 4. Addition of 30 µl cell suspension in medium + serum, 5. After 6 hours addition of 140 µl medium + serum. |
| References: |
| |