What is the effect of using amounts of input material per sample that fall outside the recommended range?

Using input amounts outside the range of 2.5 x 105 and 2.5 x 106 human or mouse cells (or the equivalent of 1.5–15 µg DNA) per sample risks affecting the quality of the resulting NGS library, such that it is likely to have higher levels of inward-facing (FR) sequence read bias, unligated ends, and read pairs containing contiguous, religated restriction fragments.
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