QIAvac Multiwell

DirectPrep 96 plates와 MinElute 96 UF PCR Purification plates용 Vacuum manifold

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QIAvac Multiwell

Cat. No. / ID: 9014579

Vacuum manifold for processing 96-well purification plates of SBS standard

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✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

The QIAvac Multiwell is designed for efficient processing of 96-well purification plates of Society for Biomolecular Screening (SBS) standard, which includes the plates contained in the DirectPrep 96 Miniprep Kit and the MinElute 96 UF PCR Purification Kit. The 96-well purification plate fits precisely into the QIAvac Multiwell top. Samples and buffers are efficiently drawn by vacuum pressure through the purification plate membrane into the collection vessel or waste tray in the QIAvac Multiwell base. The use of the QIAvac Multiwell enables parallel sample processing and reduces sample handling. Internal components are easily cleaned by simply separating top plate and base.

Resources

For use with DirectPrep 96 Miniprep Kit and MinElute 96 UF PCR Purification Kit

FAQ

We do not recommend using a vacuum manifold for DNA extraction using the QIAamp 96 DNA Blood Kit because:

Viscosity of the lysates varies from sample to sample. Therefore, the wells of the QIAamp 96 plate are at great risk of clogging.
Use of vacuum manifold would result in incomplete removal of ethanol and salts after washing with Buffer AW2. This will affect the purity and yield of samples.

FAQ ID -9158

Please contact Nalgene for ordering. Part numbers from Nalgene:

- 10 liter waste container (Nalgene Heavy Duty Vacuum Carboy [PP], cat. no. 2226-0020)

- 20 liter waste container (Nalgene Heavy Duty Vacuum Carboy [PP], cat. no. 2226-0050)

- Quick filling/venting closure (Nalgene, cat. no. 2158-0021) [lid]

- Vacuum tubing (e.g., Nalgene 180 clear plastic vacuum tubing, cat. no.8000-0065)

FAQ ID -9156

VacConnectors prevent cross-contamination and are meant to be disposed after single use.

FAQ ID -9152

Yes, the waste bottles of the QIAvac Connecting System can be autoclaved. However, please be aware that the plastic may change in color.

FAQ ID -9153

We do not recommend using a vacuum manifold for DNA extraction of tissues using the DNeasy Blood & Tissue Kit. Viscosity of the lysates varies from sample to sample; therefore, the DNeasy mini spin columns are at great risk of clogging.

FAQ ID -9154

We do not recommend to autoclave the QIAvac 24 Plus manifold. Doing so can result in damage to the device.

FAQ ID -9151

We do not recommend using a vacuum manifold for DNA extraction with the DNeasy 96 Blood & Tissue Kit because:

Viscosity of the lysates varies from sample to sample. Therefore, the wells of the DNeasy 96 plate are at great risk of clogging.
Use of vacuum manifold would result in incomplete removal of ethanol and salts after washing with Buffer AW2. This will affect the purity and yield of samples.

FAQ ID -9157

QIAvac Multiwell can hold plates that conform to SBS plate standards, whereas the QIAvac 96 does not. Therefore, the waste tray for QIAvac Multiwell is not the same as for our QIAvac 96.

However, the waste tray from QIAvac Multiwell will fit the QIAvac 96, but not vice versa.

FAQ ID -9159

We do not have this part available for replacement. You will need to replace your vacuum manifold. Please contact your Sales representative for a price quote.

FAQ ID -9161

FAQ ID -9160

We do not recommend using a vacuum manifold for DNA extraction of tissues using the QIAamp Blood & Tissue Kit. Viscosity of the lysates varies from sample to sample; therefore, the DNeasy mini spin columns are at great risk of clogging.

FAQ ID -9155

The Elution Microtube adapter of the DirectPrep 96 Miniprep Kit is an adapter needed to securely hold a rack of Elution Microtubes RS in the QIAvac Multiwell vacuum manifold. You can order the Elution Microtube adapter using part number 9236778.

The Microplate adapter is required to secure 96-well microplates in the QIAvac Multiwell vacuum manifold. The Microplate adapter can be ordered using part number 9236850.

FAQ ID -646

The filter for QIAvac Connecting System is from Pall (part no. 4400). This is the Acro 50 filter, 0.2 µm PTFE, 1/8 inch MNPT connection. Please visit www.pall.com.

FAQ ID -9150

No. The DirectPrep 96 plate of the DirectPrep 96 Miniprep Kit cannot be securely positioned in the QIAvac 96 vacuum manifold, and the nozzles may not fit into the correct wells below. Furthermore, adapters are needed to position Elution Microtubes or Microplates in the correct height for elution. If the distance of the "receiving" plate to the nozzles is not optimal, splashing and thus cross contamination can occur. Due to these difficulties with fitting, tightness and potential cross contamination, we do not recommend using the QIAvac 96 with the DirectPrep 96 System.

We recommend to use the QIAvac Mulitwell vacuum manifold in combination with the DirectPrep 96 Miniprep Kit.

FAQ ID -722