ipsogen BCR-ABL1 mbcr Kit

The ipsogen BCR-ABL1 mbcr Kit is intended for research use only and is not for use in diagnostic procedures. The kit is for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. The kit provides reagents optimized for reliable and sensitive detection and quantification of BCR-ABL mbcr p190 e1a2 transcripts, relative to ABL control gene expression, in total RNA.

To ensure highest sensitivity, the ipsogen BCR-ABL1 mbcr Kit has been optimized to detect BCR-ABL mbcr p190 e1a2 and ABL transcripts and uses prediluted plasmid standards and primers and probe mixes (see figures  Accurate detection of BCR-ABL mbcr plasmid standards and  Reliable detection of ABL plasmid standards).

The ipsogen BCR-ABL1 mbcr Kit is a ready-to-use kit for the detection of BCR-ABL mbcr p190 e1a2 fusion gene transcripts using real-time PCR. The kit is based on the amplification and detection of specific BCR-ABL mbcr p190 e1a2 transcripts, relative to ABL control gene expression, in total RNA (see figures  BCR-ABL mbcr fusion gene transcript and  ABL control gene transcript). The kit provides high levels of specificity, sensitivity, and reproducibility. The ipsogen BCR-ABL1 mbcr Kit provides 3 standard dilutions for ABL and 5 standard dilutions for BCR-ABL mbcr. Use of the standards enables accurate quantification of transcripts. The technology used for quantification of BCR-ABL mbcr has been standardized according to EAC (Europe Against Cancer) recommendations and the ipsogen BCR-ABL1 mbcr Kit uses this validated technology to calibrate results.

The first step is reverse transcription of total RNA in a sample into cDNA. The second step is the amplification of cDNA by real-time PCR. Using the ipsogen BCR-ABL1 mbcr Kit allows detection and quantification of BCR-ABL mbcr p190 e1a2 and ABL transcripts. Simply start the reaction using the optimized protocols described in the kit handbook.

The ipsogen BCR-ABL1 mbcr Kit enable sensitive and reliable detection and quantification of BCR-ABL mbcr p190 e1a2 transcripts, relative to ABL control gene expression, for research use only.