Ultrafiltration이 포함된 96-well format으로 PCR products (100 bp이상)를 high-throughput으로 분리 정제할 수 있습니다
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MinElute 96 UF PCR Purification Kit (4)
Cat. No. / ID: 28051
4 MinElute 96 UF PCR Purification Plates
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Pieces
4
24
MinElute 96 UF PCR Purification Kit은/는 분자생물학 분야에 사용하기 위한 것입니다. 이 제품은 질병의 진단, 예방, 또는 치료용이 아닙니다.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- 극소량의 elution volume
- 빠른 처리과정과 쉬운 핸들링
- High, reproducible recoveries
Product Details
The MinElute 96 UF PCR Purification Kit provides 96-well plates for high-throughput ultrafiltration-based purification of PCR products >100 bp in size. The plates are designed to allow elution in very small volumes (as little as 20 μl) delivering high yields of highly concentrated DNA. The procedure can be automated on the BioRobot Universal System. DNA fragments purified with the MinElute system are ready for direct use in many applications, including sequencing, microarray analysis, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription. The MinElute 96 UF PCR Purification Kit requires the use of the QIAvac Multiwell vacuum manifold (cat. no. 9014579).
Performance
The MinElute 96 UF PCR Purification Kit provides high-throughput PCR purification in 96-well plates. Fully automated processing is provided for 1–8 x 96 samples on the BioRobot 3000 (no longer available), the BioRobot 8000, or the BioRobot Universal System, or for 1 x 96 samples on the QIAvac Multiwell Manifold (cat. no. 9014579, requires suitable vacuum manifold, e.g., QIAvac Multiwell manifold, plate shaker recommended). The procedure purifies DNA >100 bp in size with a recovery of 80–95%. Purified PCR products are eluted directly from the surface of the membrane in small volumes (as little as 20 µl in the manual procedure or 30 µl on BioRobot workstations), providing high-concentration eluates (see figure " Highly concentrated DNA eluates") that are suitable for direct use in a variety of downstream applications. It is possible to process PCR sample volumes up to 150 µl using either a manual or fully automated procedure.
See figures
Principle
The QIAGEN MinElute 96 UF PCR Purification Kit combines the convenience of multiwell technology and the separation properties of an ultrafiltration membrane with a unique design. The plates enable the fast and efficient recovery of double-stranded DNA fragments >100 bp and the removal of primers (<20mers), unincorporated nucleotides, and salts.
Procedure
MinElute 96 UF PCR Purification Kits provide ultrafiltration technology using vacuum-driven purification on QIAvac manifolds (see flowchart " MinElute 96 UF PCR Purification procedure"). PCR products are loaded into the wells of the ultrafiltration plates, and a vacuum is applied. While small molecules such as primers, salts, and unincorporated nucleotides pass through the membrane, PCR products >100 bp are retained. Water, DMSO, or buffers such as SSC can be used for elution. The MinElute 96 UF PCR Purification procedure can also be automated on the BioRobot 3000 (no longer available), the BioRobot 8000, or the BioRobot Universal System.
See figures
Applications
DNA fragments purified with the MinElute 96 UF system are ready for direct use in many applications, including:
- Sequencing
- Microarray analysis
- Ligation and transformation
- Restriction digestion
- Labeling
Supporting data and figures
miScript miRNA QC PCR Array layout for plate formats E and G.
miScript miRNA QC PCR Array layout for plate formats E and G.
Specifications
| Features | Specifications |
|---|---|
| Binding capacity | <150 µl |
| Recovery: oligonucleotides dsDNA | Recovery: oligonucleotides, dsDNA |
| Elution volume | 20–30µl |
| Format | 96-well plate |
| Fragment size | >100 bp |
| Removal <10mers 17–40mers dye terminator proteins | Removal <20mers |
| Technology | Ultrafiltration |
| Sample type: applications | DNA, oligonucleotides: PCR reactions |
| Processing | Manual/automated |
Resources
Kit Handbooks (1)
User-Developed Protocols (1)
Quick-Start Protocols (1)
Certificates of Analysis (1)
FAQ
Can MinElute 96 UF PCR be used to purify PCR samples which contain mineral oil?
Can I use a partial MinElute 96 UF plate?
Do you have a protocol for concentration of RNA in 96-well plates?