Stool에서 genomic, bacterial, viral, parasite DNA를 30 ug까지 분리 정제할 수 있습니다
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QIAamp Fast DNA Stool Mini Kit (50)
Cat. No. / ID: 51604
For 50 DNA preps: 50 QIAamp Mini Spin Columns, QIAGEN Proteinase K, InhibitEX Buffer, wash and elution buffers, Collection Tubes (2 ml)
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KitBuffer
QIAamp Fast DNA Stool Mini Kit
Inhibitex Buffer
QIAamp Fast DNA Stool Mini Kit은/는 분자생물학 분야에 사용하기 위한 것입니다. 이 제품은 질병의 진단, 예방, 또는 치료용이 아닙니다.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- High-quality의 신속한 분리 정제, ready-to use DNA
- Organic extraction 또는 alcohol precipitation 불필요
- 효율이 높고 결과가 일정함
- 정확한 downstream applications을 위해 inhibitor와 contaminant를 완벽히 제거함
Product Details
The QIAamp DNA Stool Mini Kit provides silica membrane-based purification of up to 30 μg genomic, bacterial, viral, and parasite DNA from fresh or frozen human stool or other sample types with high concentrations of PCR inhibitors. The combined action of InhibitEX, a unique adsorption resin, and an optimized buffer leads to removal of PCR inhibitors. The convenient QIAamp spin-column procedure provides purification in only 50 minutes. Purification of DNA using the QIAamp DNA Stool Mini Kit can be automated on the QIAcube.
Performance
The QIAamp DNA Stool Mini Kit allows rapid purification of DNA from fresh or frozen human stool or other sample types with high concentrations of PCR inhibitors. Up to 220 mg stool can be processed routinely, and larger amounts can be processed with additional Buffer ASL. Typical yields of 10–30 µg are obtained in 50 minutes, and DNA is eluted in 200 µl. The purified DNA is sized up to 50 kb. DNA of this length denatures completely and has the highest amplification efficiency. Highly pure DNA is ready for direct use in downstream amplification reactions (see figure "Removal of PCR inhibitors").
DNA that has been purified using the QIAamp DNA Stool Mini Kit can be used in a wide range of downstream applications, including PCR and quantitative real-time PCR, infectious disease research, and screening.
Principle
DNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. No phenol–chloroform extraction is required. PCR inhibitors are removed by the combined action of InhibitEX, a unique adsorption resin, and an optimized buffer. Rigorous lysis using proteinase K ensures high yields of all types of DNA common in stool, including colorectal epithelial cells, bacteria, viruses and other gastrointestinal pathogens.
Highly pure DNA ready for direct use in downstream amplification reactions is purified in about 50 minutes. QIAamp sample preparation technology is fully licensed.
Procedure
The QIAamp DNA Stool Mini Kit simplifies isolation of DNA from stool with a fast spin-column procedure (see flowchart "Procedure"). Optimized buffers and enzymes lyse samples, stabilize nucleic acids, and enhance selective DNA adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp spin column. PCR inhibitors are removed by the combined action of InhibitEX and an optimized buffer. Proteinase K lysis ensures high yields of all types of DNA common in stool. Remaining impurities are efficiently removed in two wash steps. Amplification-ready DNA is then eluted in low-salt buffer.
Applications
The QIAamp DNA Stool Mini Kit is for isolation of genomic, bacterial, viral, and parasite DNA from the following sample types:
- Fresh stool
- Frozen stool
- Other sample types with high concentrations of PCR inhibitors
Supporting data and figures
Superior DNA yields compared to similar kits.
DNA was purified from 7 stool samples using the QIAamp Fast DNA Stool Mini Kit and solutions from 2 alternative suppliers. Yields were measured by UV spectrophotometry. Yields were invariably highest with the QIAamp Fast DNA Stool Mini Kit.
Resources
Kit Handbooks (1)
Safety Data Sheets (3)
Brochures & Guides (1)
Quick-Start Protocols (1)
Certificates of Analysis (1)
FAQ
What is the composition of elution buffer ATE in the QIAamp DNA Investigator kit, QIAamp DNA FFPE Tissue kit and the QIAamp Fast DNA Stool Mini kit?