BioRobot M48 workstation을 이용하여 300 ul serum 혹은 plasma 샘플에서 viral RNA를 자동으로 분리 정제합니다
Products
MagAttract Viral RNA M48 Kit은/는 분자생물학 분야에 사용하기 위한 것입니다. 이 제품은 질병의 진단, 예방, 또는 치료용이 아닙니다.
MagAttract Viral RNA Kit (960)
Cat. No. / ID: 955538
Automated virus preparation on a third-party platform (KingFisher Flex). Proteinase K and buffers for offboard lysis, MagAttract Suspension G beads and buffers for onboard bind, wash and elute steps.
MagAttract Viral RNA Kit (960)은/는 분자생물학 분야에 사용하기 위한 것입니다. 이 제품은 질병의 진단, 예방, 또는 치료용이 아닙니다.
Features
- 신속한 분리 정제 — high-quality viral RNA
- Flexible purification of 6–48 samples per run
- 50, 65, 80, 100 ul의 elution volumn으로 RNA농도를 최적화하기 쉬움
Product Details
The MagAttract Viral RNA M48 Kit provides automated purification of viral RNA on the BioRobot M48 using proven MagAttract magnetic particle technology. Variable elution volumes enable easy optimization of viral RNA concentration for downstream applications. Viral RNA purification and magnetic separation take place in the pipet tips, increasing the efficiency of the procedure.
Performance
The yield of viral RNA per sample with the MagAttract Viral RNA M48 Kit is normally below 1 µg, and is therefore difficult to determine with a spectrophotometer. Quantitative amplification methods are recommended for determination of yields.
Principle
The MagAttract Viral RNA M48 Kit provides fully automated purification of viral RNA from serum and plasma. MagAttract technology combines the speed and efficiency of silica-based RNA purification with the convenient handling of magnetic particles. Samples are processed in the BioRobot M48 in 1.5 ml or 2 ml sample tubes. Up to 48 samples in increments of 6 samples can be processed per run. The sample source can be up to 300 µl serum or plasma. The yield of purified RNA depends on the sample type and virus titer.
Procedure
In the MagAttract Viral RNA M48 procedure, viral RNA binds to the silica surface of the magnetic particles in the presence of a chaotropic salt. Viral RNA bound to the particles is then efficiently washed using two different wash buffers. High-quality viral RNA is eluted at 65ºC in an RNase-free elution buffer.
Applications
The high-quality RNA obtained using the MagAttract Viral RNA M48 Kit and the BioRobot M48 is suited for use in many downstream applications, such as:
- Viral epidemiology and genotyping
- Infectious disease research
Supporting data and figures
Recovery of SARS-CoV-2 RNA from respiratory swab supernatants.
RNA purified from respiratory swab supernatant using the indicated kits was subjected to RT-PCR (mean value and deviation of threshold cycle shown). (A) negative samples spiked with AccuPlex SARS-CoV-2; (B) 1:10 diluted SARS-CoV-2 positive samples.
Specifications
| Features | Specifications |
|---|---|
| ApplicationsKO | PCR, real-time PCR, digital PCR, genotyping, sequencing |
| Sample amount | 300 µl |
| For automated processing | KingFisher Flex Workdeck |
| Elution volume | 80 µl |
| Main sample type | Respiratory swabs |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Viral RNA |
| Processing | Automated |
| Format | 96 well |
| Technology | Magnetic particles |
Resources
Quick-Start Protocols (1)
Kit Handbooks (1)
Safety Data Sheets (2)
Certificates of Analysis (1)
FAQ
Where can I download the protocol for the KingFisher Flex?
Who can help if it is not clear if issues are caused by the third party instrument or by the chemistry?
Who can help in case of issues with the third party instrument?
Is the virus inactivated during the procedure?
I would like to use the kit on another third party instrument. Who should I contact?
Can I use sample volumes other than 300 µl?
Are protocols also available for other instruments (e.g., Hamilton Star and Tecan Fluent)?