Single tube에 있는 whole blood, buffy coat과 cultured cell에서 genomic DNA를 42 µg까지 분리할 수 있습니다
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FlexiGene DNA Kit (250)
Cat. No. / ID: 51206
For isolation of genomic DNA from whole blood, buffy coat, and cultured cells in a single tube with fast kit preparation
FlexiGene DNA Kit은/는 분자생물학 분야에 사용하기 위한 것입니다. 이 제품은 질병의 진단, 예방, 또는 치료용이 아닙니다.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- 쉬운 사용방법 — 샘플 섞이는 위험성이 없음
- 1–20 ml의 whole blood에서 분리 정제, starting material의 양에 따른 flexibility
- 배양한 세포, buffy coat, 전혈로 부터 바로 분리 정제함
- 빠른 처리과정
- organic extraction 불필요
Product Details
The FlexiGene system uses simple centrifugation to isolate highly pure DNA from whole blood, buffy coat, and cultured cells. The FlexiGene DNA Kit contains buffers and QIAGEN Protease for direct, simple purification in a single tube. For automated solutions using FlexiGene reagents, contact Autogen, Inc.
Performance
DNA purified using the FlexiGene system is sized up to 150 kb (see figure " High-quality genomic DNA"). It performs well in amplification and Southern blotting experiments, and is also suitable for archiving (see figure " Efficient downstream PCR analysis").
DNA is purified from 0.1–20 ml (blood), 0.1–2 ml (buffy coat), or 1–2 x 106 cells (cultured cells) in a single tube, processed by centrifugation. Twelve samples can be processed in 1 hour. Depending on sample size, the rehydration volume is 0.2–1 ml.
DNA yields purified from different sample types using the FlexiGene procedure Sample Volume DNA yield (µg) Blood* 0.3 ml 11–14 Blood* 2.0 ml 75–90 Blood* 10.0 ml 380–460 Buffy coat 0.5 ml 110–130 HeLa cells 106 cells 25–35 * Whole blood with normal white blood cell counts (7x106 cells/ml blood).
| Sample | Volume | DNA yield (µg) |
|---|---|---|
| Blood* | 0.3 ml | 11–14 |
| Blood* | 2.0 ml | 75–90 |
| Blood* | 10.0 ml | 380–460 |
| Buffy coat | 0.5 ml | 110–130 |
| HeLa cells | 106 cells | 25–35 |
See figures
Principle
The FlexiGene DNA system enables isolation of genomic DNA from variable amounts of whole blood, buffy coat, and cultured cells. The simple purification procedure is performed in a single tube, which reduces costs and simplifies handling. The purified DNA performs well in sensitive downstream applications.
Procedure
The first step is addition of lysis buffer to the samples (see flowchart " FlexiGene DNA procedure"). Cell nuclei and mitochondria are pelleted by centrifugation and resuspended in denaturation buffer containing QIAGEN Protease. Following protein digestion, DNA is precipitated by addition of isopropanol, recovered by centrifugation, washed in 70% ethanol, and dried. DNA is resuspended in hydration buffer and is ready for direct use in downstream assays or storage at –20ºC. High DNA yields are obtained from whole blood, buffy coat, and cultured cells (see table "DNA yields purified from different sample types using the FlexiGene procedure").
See figures
Applications
DNA purified using the FlexiGene DNA kit performs well downstream applications, including,
- PCR
- Southern blotting
- Archiving
Supporting data and figures
FlexiGene DNA procedure.
The first step is addition of lysis buffer to the samples. Cell nuclei and mitochondria are pelleted by centrifugation and resuspended in denaturation buffer containing QIAGEN Protease. Following protein digestion, DNA is precipitated by addition of isopropanol, recovered by centrifugation, washed in 70% ethanol, and dried. DNA is resuspended in hydration buffer and is ready for direct use in downstream assays or storage at –20ºC.
Specifications
| Features | Specifications |
|---|---|
| ApplicationsKO | PCR techniques, restriction digest, Southern blotting, sequencing |
| Sample amount | 100 µl - 20 ml |
| Format | Scalable |
| Time per run or per prep | 25 - 45 minutes (+ DNA rehydration) |
| Main sample type | Whole blood, buffy coat, cultured cells |
| Technology | Single tube precipitation method |
| Processing | Manual |
| Yield | 35 µg / ml whole blood |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Genomic DNA |
| Elution volume | 200 µl - 1000 µl |
Resources
Kit Handbooks (1)
Supplementary Protocols (1)
Additional Resources (1)
Safety Data Sheets (2)
Certificates of Analysis (1)
FAQ
Do you have a protocol for purification of DNA from clotted blood using the FlexiGene DNA Kit?
Do you have stability data for genomic DNA isolated with the Flexigene DNA Kit?
Do you have a protocol for the isolation of genomic DNA from frozen clotted blood?
What is the composition of Buffer FG3?