지방이 많은 조직에서 total RNA 100 ug까지 분리 정제할 수 있습니다
✓ 연중무휴 하루 24시간 자동 온라인 주문 처리
✓ 풍부한 지식과 전문성을 갖춘 제품 및 기술 지원
✓ 신속하고 안정적인 (재)주문
RNeasy Lipid Tissue Mini Kit (50)
카탈로그 번호 / ID. 74804
50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, RNase-free Reagents and Buffers
로그인 계정 가격 보기.
RNeasy Lipid Tissue Mini Kit은/는 분자생물학 분야에 사용하기 위한 것입니다. 이 제품은 질병의 진단, 예방, 또는 치료용이 아닙니다.
✓ 연중무휴 하루 24시간 자동 온라인 주문 처리
✓ 풍부한 지식과 전문성을 갖춘 제품 및 기술 지원
✓ 신속하고 안정적인 (재)주문
특징
- 지방조직에 적합한 최적화된 lysis 조건
- QIAzol lysis와 RNeasy purification의 통합 — 따라하기 쉬운 protocol
- Phenol contamination 없이 고효율의 total RNA 분리
제품 세부 정보
The RNeasy Lipid Tissue Mini Kit includes QIAzol Lysis Reagent for lysing fatty tissues and other types of tissue, and RNeasy spin columns for purifying up to 100 µg of high-quality RNA. The kit can be automated using the QIAcube. Tissue samples can be conveniently stabilized using RNAlater RNA Stabilization Reagent (nonfatty tissues only) or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system. For larger samples, the RNeasy Lipid Tissue Midi Kit (spin-column binding capacity of 1 mg RNA) is also available.
성능
The RNeasy Lipid Tissue protocol integrates QIAzol lysis with RNeasy RNA isolation for high yields of total RNA from fatty tissues with an average yield of RNA from 10 mg of brain of 10 µg, and 2.4 µg of RNA from a 10 mg sample of adipose tissue. RNeasy Lipid Tissue kits provide efficient isolation of high-quality total RNA without phenol carryover (see figure " High yields of RNA without phenol carryover"). The RNA from fatty tissues is suitable for downstream applications including real-time RT-PCR (see figure " Real-time analysis of high-quality RNA").
원리
RNeasy Lipid Tissue Kits are optimized for use with fatty tissues, such as brain and adipose tissue. The convenient RNeasy Lipid Tissue protocol integrates optimized phenol/guanidine-based lysis with proven RNeasy purification for isolation of high yields of high-quality total RNA. The combination of organic extraction and chaotropic disruption contributes to the high lysis efficiency of QIAzol Lysis Reagent. Since the RNeasy procedures enrich for mRNA and other RNA species >200 nucleotides, the total RNA yield does not include 5S rRNA, tRNA, and other low-molecular-weight RNAs, which make up 15-20% of total cellular RNA.
절차
Tissue samples 10–100 mg are homogenized in QIAzol Lysis Reagent. After addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the RNeasy spin column, where the total RNA (up to 100 µg) binds to the membrane and phenol and other contaminants are efficiently washed away. High-quality RNA is then eluted in 30–100 µl of RNase-free water water (see figure " RNeasy Lipid Tissue Mini procedure").
응용 분야
RNeasy Lipid Tissue Kits provide easy and efficient isolation of high-quality RNA for all downstream applications, including array analysis and real-time RT-PCR.
지원되는 데이터 및 수치
High yields of RNA without phenol carryover.
RNA was isolated from 10 mg rat brain tissue using the RNeasy Lipid Tissue Mini Kit (RNeasy Lipid), a standard silica-gel-membrane procedure (Std. silica), or a phenol/guanidine-based reagent (PhOH/Gdn), following supplier's instruction. [A] Formaldehyde agarose gel analysis shows high yields of RNA using the RNeasy Lipid Tissue Mini Kit. Using the RNeasy and other silica-based methods, small RNAs (such as 5.8S rRNA, 5S rRNA, and tRNAs) are selectively excluded. [B] Absorbance spectrum shows contaminants and phenol when using the phenol/guanidine-based reagent.
사양
| 특징 | 사양 |
|---|---|
| Sample amount | 10–100 mg |
| Yield | 2.4–10 mg |
| Technology | Silica technology |
| Processing | Manual |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | RNA |
| Elution volume | 30–100 µl |
| Main sample type | Fatty tissue samples |
| Time per run or per prep | 45 minutes |
| Format | Spin column |
리소스
Safety Data Sheets (1)
Brochures & Guides (1)
User-Developed Protocols (4)
Kit Handbooks (1)
Quick-Start Protocols (1)
Gene Expression Analysis (1)
Certificates of Analysis (1)
출판물
Lipopolysaccharide activates an innate immune system response in human adipose tissue in obesity and type 2 diabetes.
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Chronic ethanol feeding to rats decreases adiponectin secretion by subcutaneous adipocytes.
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A study of alternative splicing in the pig.
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