Isolation of plasmid DNA from Lactobacillus spp. using the QIAGEN® Plasmid Midi Kit - (EN)
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The procedure has been used successfully for isolation of cryptic plasmids (pLC2-based) from mesophilic
Lactobacillus
strains such as
L. sake
and
L. curvatus
. Yield of plasmid DNA was typically 10-20 µg plasmid DNA from 100 ml culture.
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