For 100 x 50 µl reactions: QIAGEN OneStep RT-PCR Enzyme Mix (1 x 200 µl), 5x QIAGEN OneStep RT-PCR Buffer (1 x 1 ml), dNTP Mix (1 x 200 µl, 10 mM each), 5x Q-Solution (1 x 2 ml), RNase-Free Water (2 x 1.9 ml)

Alignment marker with 15 bp and 3 kb fragments

For the isolation of microbial DNA from stool and gut samples

DNA size marker with fragments of 100, 200, 300, 400, 500, 600, 700, 800, 1000, 1200, 1500, 2000, and 2500 bp; concentration 100 ng/µl

50 DNeasy Mini Spin Columns, Proteinase K, Buffers, Collection Tubes (2 ml)

250 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers.

For DNA fragment purification from PCR, gel fragments and enzymatic reactions to use with the buffer leftovers of the QIAquick Nucleotide Removal Kit

1500 Kunitz units RNase-free DNase I, RNase-free Buffer RDD, and RNase-free water for 50 RNA minipreps

For the isolation of microbial genomic DNA from all soil types.

For 50 DNA minipreps: 50 QIAamp Mini Spin Columns, QIAGEN Protease, Reagents, Buffers, Collection Tubes (2 ml)

50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers

250 DNeasy Mini Spin Columns, Proteinase K, Buffers, Collection Tubes (2 ml)

For 50 minipreps: RNeasy Mini Spin Columns, gDNA Eliminator Spin Columns, Collection Tubes, RNase-Free Water and Buffers

50 DNeasy Mini Spin Columns, 50 QIAshredder Mini Spin Columns, RNase A, Buffers, Collection Tubes (2 ml)

DNA size marker with fragments of 50, 100, 150, 200, 250, 300, 400, 500, 600, 700, and 800 bp; concentration 100 ng/ul

Disposable Filter-Tips, racked; (8 x 128). For use with the QIAcube

200 ml QIAzol Lysis Reagent