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ipsogen Leukemia
主页
产品
Diagnostics & Clinical Research
Oncology
ipsogen Leukemia
ipsogen
JAK2...
ipsogen
JAK2 Muta
Search
Kit
采用real-time PCR对JAK2 V617F/G1849T突变进行定量检测
特点
灵敏、准确的定量检测试剂
能够检测1%临界值的阳性样本
即用型解决方案,便利的工作流程
产品详情
ipsogen
JAK2 Muta
Search
Kit仅适用于科研,不可用于诊断。该试剂盒可用于Rotor-Gene Q实时荧光定量PCR分析仪或其他real-time PCR仪的real-time PCR实验。该试剂盒中的试剂经过优化,能够对基因组DNA中的JAK2 V617F/G1849T突变进行灵敏、可靠的检测。
绩效
为确保检测的高灵敏度,
ipsogen
JAK2 Muta
Search
Kit已经过优化,利用1%的DNA临界样本(COS)、阳性和阴性对照,检测JAK2 V617F/G1849T突变(参见
Accurate detection of JAK2 V617F mutation, 1% cut-off sample, and positive and negative controls
和
Reliable detection of wild-type JAK2, 1% cut-off sample, and positive and negative controls
)。推荐的起始样本DNA最少为25 ng。
原理
ipsogen
JAK2 Muta
Screen
Kits为即用型试剂盒,能够对基因组DNA中SNP导致的JAK2 V617F/G1849T体细胞突变进行检测分析(参见
JAK2 mutation site identification
)。该试剂盒灵敏度高,可重复性强。
ipsogen
JAK2 Muta
Screen
Kits提供JAK2 V617F的DNA检测临界样本(COS)、DNA阳性对照(PC)和阴性对照(NC)。使用检测临界样本和对照能够实现准确的基因分型分析。
程序
第一步是抽提总DNA,并检测其量与品质。第二步是通过real-time PCR扩增DNA。
ipsogen
JAK2 Muta
Search
Kits能够对JAK2 V617F/G1849T突变进行灵敏的定量检测。只需按照试剂盒操作手册中的实验方案,即可便利的开始反应。
应用
ipsogen
JAK2 Muta
Search
Kits能够对JAK2 V617F/G1849T突变进行灵敏、可靠的定量检测,仅适用于科研。
辅助数据和图表
JAK2 mutation site identification.
JAK2 mutation site identification.
资源