RNeasy Lipid Tissue Mini Kit

지방이 많은 조직에서 total RNA 100 ug까지 분리 정제할 수 있습니다

✓ 연중무휴 하루 24시간 자동 온라인 주문 처리

✓ 풍부한 지식과 전문성을 갖춘 제품 및 기술 지원

✓ 신속하고 안정적인 (재)주문

RNeasy Lipid Tissue Mini Kit (50)

카탈로그 번호 / ID. 74804

50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, RNase-free Reagents and Buffers

문의

RNeasy Lipid Tissue Mini Kit은/는 분자생물학 분야에 사용하기 위한 것입니다. 이 제품은 질병의 진단, 예방, 또는 치료용이 아닙니다.

✓ 연중무휴 하루 24시간 자동 온라인 주문 처리

✓ 풍부한 지식과 전문성을 갖춘 제품 및 기술 지원

✓ 신속하고 안정적인 (재)주문

특징

지방조직에 적합한 최적화된 lysis 조건
QIAzol lysis와 RNeasy purification의 통합 — 따라하기 쉬운 protocol
Phenol contamination 없이 고효율의 total RNA 분리

제품 세부 정보

The RNeasy Lipid Tissue Mini Kit includes QIAzol Lysis Reagent for lysing fatty tissues and other types of tissue, and RNeasy spin columns for purifying up to 100 µg of high-quality RNA. The kit can be automated using the QIAcube. Tissue samples can be conveniently stabilized using RNAlater RNA Stabilization Reagent (nonfatty tissues only) or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system. For larger samples, the RNeasy Lipid Tissue Midi Kit (spin-column binding capacity of 1 mg RNA) is also available.

성능

The RNeasy Lipid Tissue protocol integrates QIAzol lysis with RNeasy RNA isolation for high yields of total RNA from fatty tissues with an average yield of RNA from 10 mg of brain of 10 µg, and 2.4 µg of RNA from a 10 mg sample of adipose tissue. RNeasy Lipid Tissue kits provide efficient isolation of high-quality total RNA without phenol carryover (see figure ""). The RNA from fatty tissues is suitable for downstream applications including real-time RT-PCR (see figure "").

원리

RNeasy Lipid Tissue Kits are optimized for use with fatty tissues, such as brain and adipose tissue. The convenient RNeasy Lipid Tissue protocol integrates optimized phenol/guanidine-based lysis with proven RNeasy purification for isolation of high yields of high-quality total RNA. The combination of organic extraction and chaotropic disruption contributes to the high lysis efficiency of QIAzol Lysis Reagent. Since the RNeasy procedures enrich for mRNA and other RNA species >200 nucleotides, the total RNA yield does not include 5S rRNA, tRNA, and other low-molecular-weight RNAs, which make up 15-20% of total cellular RNA.

절차

Tissue samples 10–100 mg are homogenized in QIAzol Lysis Reagent. After addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the RNeasy spin column, where the total RNA (up to 100 µg) binds to the membrane and phenol and other contaminants are efficiently washed away. High-quality RNA is then eluted in 30–100 µl of RNase-free water water (see figure "").

응용 분야

RNeasy Lipid Tissue Kits provide easy and efficient isolation of high-quality RNA for all downstream applications, including array analysis and real-time RT-PCR.

지원되는 데이터 및 수치

High yields of RNA without phenol carryover.

RNA was isolated from 10 mg rat brain tissue using the RNeasy Lipid Tissue Mini Kit (RNeasy Lipid), a standard silica-gel-membrane procedure (Std. silica), or a phenol/guanidine-based reagent (PhOH/Gdn), following supplier's instruction. [A] Formaldehyde agarose gel analysis shows high yields of RNA using the RNeasy Lipid Tissue Mini Kit. Using the RNeasy and other silica-based methods, small RNAs (such as 5.8S rRNA, 5S rRNA, and tRNAs) are selectively excluded. [B] Absorbance spectrum shows contaminants and phenol when using the phenol/guanidine-based reagent.

사양

특징	사양
Sample amount	10–100 mg
Yield	2.4–10 mg
Technology	Silica technology
Processing	Manual
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	RNA
Elution volume	30–100 µl
Main sample type	Fatty tissue samples
Time per run or per prep	45 minutes
Format	Spin column

리소스

Download Safety Data Sheets for QIAGEN product components.

출판물

Lipopolysaccharide activates an innate immune system response in human adipose tissue in obesity and type 2 diabetes.

Creely SJ; McTernan PG; Kusminski CM; Fisher fM; Da Silva NF; Khanolkar M; Evans M; Harte AL; Kumar S;

Am J Physiol Endocrinol Metab; 2006; 292 (3):E740-7 2006 Nov 7 PMID:17090751

Chronic ethanol feeding to rats decreases adiponectin secretion by subcutaneous adipocytes.

Chen X; Sebastian BM; Nagy LE;

Am J Physiol Endocrinol Metab; 2006; 292 (2):E621-8 2006 Oct 17 PMID:17047161

Targeting an E2F site in the mouse genome prevents promoter silencing in quiescent and post-mitotic cells.

Tavner F; Frampton J; Watson RJ;

Oncogene; 2006; 26 (19):2727-35 2006 Oct 30 PMID:17072340

Paternally biased X inactivation in mouse neonatal brain.

Wang X; Soloway PD; Clark AG;

Genome Biol; 2010; 11 (7):R79 2010 Jul 27 PMID:20663224

A study of alternative splicing in the pig.

Nygard AB; Cirera S; Gilchrist MJ; Gorodkin J; Jørgensen CB; Fredholm M;

BMC Res Notes; 2010; 3:123 2010 May 5 PMID:20444244

FAQ

RNeasy Mini, Midi and Maxi Kits should be stored dry at room temperature (15 to 25°C). The RNeasy MinElute Spin Columns of the RNeasy Micro Kit and RNeasy MinElute Cleanup Kit should be stored at 4°C. RNeasy Kits are stable for at least 9 months under these conditions.

FAQ-103

Our RNeasy buffers are subjected to stringent quality-control tests to ensure that they are indeed RNase-free. Buffer RPE concentrate and RNase-free water are tested for absence of RNases by incubating 4 µg of total HeLa-RNA in these solutions for 3 hours at 37°C, followed by monitoring RNA integrity via denaturing agarose gel electrophoresis and ethidium bromide staining.

Buffer RLT and Buffer RW1 are inherently RNase-free, since the buffers themselves inactivate RNases during the RNeasy procedure.

FAQ-113

Disruption:

Complete disruption of cell walls and plasma membranes of cells and organelles is absolutely required to release all RNA contained in a sample. Different samples require different methods to achieve complete disruption. Please refer to the section 'Disruption and homogenization of starting materials' in the RNeasy Mini Handbook. Incomplete disruption results in significantly reduced RNA yields.

Homogenization:

Homogenization is necessary to reduce the viscosity of the cell lysates produced by disruption. Homogenization shears the high-molecular-weight genomic DNA and other high-molecular-weight cellular components to create a homogeneous lysate. Incomplete homogenization results in genomic DNA contamination, and inefficient binding of RNA to the RNeasy membrane resulting in reduced yields.

FAQ-139

At this time, RNeasy spin columns are not sold separately.

FAQ-159

The maximum binding capacity of the RNeasy Mini spin columns is 100 ug RNA. It is 1 mg for RNeasy Midi columns and 6 mg for RNeasy Maxi columns. The maximum RNA binding capacity of the RNeasy MinElute spin columns is 45 µg.

FAQ-290

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