Rotor-Disc 100 Starter Kit

配合Rotor-Gene Q实时荧光定量PCR分析仪使用

S_1084_5_GEN_V2
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Rotor-Disc 100 Starter Kit (115V)

目录编号 / ID.   9001602

3 Rotor-Disc 100 packs for 15–25 µl reaction volumes, Rotor-Disc Heat Sealer, Rotor-Disc Heat Sealing Film, Rotor-Disc 100 Rotor and Locking Ring, Rotor-Disc Pipetting Aid, Rotor-Disc Easy Lift Adaptor
Product
Rotor-Disc 100 Starter Kit (115V)
Rotor-Disc 100 Starter Kit (230V)
The Rotor-Disc 100 Starter Kit is for use with the Rotor-Gene Q thermal cycler.
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特点

  • 加快反应体系构建,提高通量
  • 配合Rotor-Gene Q实时荧光定量PCR分析仪进行real-time PCR,表现卓越

产品详情

Rotor-Disc 100 Starter Kit提供在Rotor-Gene Q实时荧光定量PCR分析仪上进行100个反应所需的所有试剂和耗材。

原理

Rotor-Disc 100 Starter Kit为Rotor-Disc 100孔盘规格,可在Rotor-Gene Q实时荧光定量PCR分析仪上便利的进行real-time PCR。该试剂盒含有Rotor-Disc 100孔盘、Rotor-Disc Heat Sealer、Rotor-Disc Heat Sealing Film、Rotor-Disc 100 Rotor和Locking Ring、Rotor-Disc 100 Loading Block、以及Rotor-Disc Pipetting Aid。

Rotor-Discs与QIAgility仪器兼容,因此反应体系构建可自动进行。

程序

Rotor-Disc 100 Rotor和Rotor-Disc 100 Locking Ring与Rotor-Disc 100孔盘配套使用。Rotor-Disc 100孔盘为圆形,有100个垂直的反应孔,适合高通量应用。Rotor-Disc 100孔盘相当于圆形的96孔板,外加4个对照孔。因此,平时使用96孔板进行实验的实验室可以便利的用Rotor-Gene Q实时荧光定量PCR分析仪进行操作。多出的孔可放置更多样本、对照,不会占据96孔。为无缝整合到96孔板工作流程中,Rotor-Disc 100孔盘使用96孔板的标记方法,如:从A1-A12到H1-H12。多出的4个孔被标记为R1-R4。

Rotor-Disc 100孔盘无需管盖。只需将Rotor-Disc Heat Sealing Film置于孔的顶部,用Rotor-Disc Heat Sealer加热即可密封。牢固、结实的密封膜可避免污染。

应用

使用Rotor-Gene Q实时荧光定量PCR分析仪和Rotor-Disc 100 Starter Kit可进行real-time PCR和高分辨率熔解分析,包括:

  • 基因表达分析
  • 病原体检测
  • DNA甲基化分析
  • 基因分型
  • 基因扫描
  • miRNA研究

资源

Brochures and Guides (1)
Now with even more applications!
Protocols (1)
Calibration guide for use with QIAgility and the Rotor-Disc 100
User Manuals (1)
Rotor-Disc Heat Sealer
LITERATURE (1.2MB)
Download
Safety Data Sheets (1)
Download Safety Data Sheets for QIAGEN product components.
Certificates of Analysis (1)

常见问答

How many samples can be run in parallel with the Rotor-Gene Q real-time PCR instrument?

The Rotor-Gene Q instrument can be used with two different rotor formats: using tubes or rotor-dics. Tubes can be run in 36 and 72-well rotors and rotor-discs in Rotor-Disc 72 and Rotor-Disc 100 rotors. When programming the temperature profile please make sure the correct rotor type is selected.  

 

 

FAQ-1519
Are Rotor-Gene Kits compatible with reaction setup using the QIAgility instrument?

The majority of the Rotor-Gene Kit data shown in our literature has been generated with the help of the QIAgility instrument. We did not observe any problems during the pipetting steps.

 

FAQ-2116
Do the master mixes in Rotor-Gene Kits contain dUTP to allow UNG pretreatment?

No. The master mixes in Rotor-Gene Kits contain dTTP instead of dUTP. If UNG treatment is required, we recommend using QuantiTect +UNG Kits. QuantiTect Kits are also compatible with the Rotor-Gene Q; however, the kits require a significantly longer cycling time.

 

 

FAQ-2117
What are the main differences between Rotor-Gene and QuantiTect or QuantiFast PCR Kits?

Rotor-Gene Kits are specifically developed for the Rotor-Gene Q PCR Cycler. The unique rotary system of the cycler combined with the kits’ proprietary buffer system enable ultrafast cycling. Rotor-Gene Kits do not contain ROX dye since no normalization to a passive reference is required. Also, Rotor-Gene Kits do not contain dUTP; therefore, UNG pretreatment is not possible.

 

FAQ-2119