FAQ-2707
What do I need to complete a RT² qPCR Primer Assay?
You need:
- A RT² SYBR Green Mastermix that matches the qPCR instrument in your laboratory;
- RT² qPCR Primer Assays for your target genes;
- A Housekeeping gene RT² qPCR Primer Assay.
We also recommend using our RT² First Strand Kit for reverse transcription.
Related products
RT2 SYBR® Green qPCR Mastermixes
RT2 SYBR Green qPCR Mastermixes are highly suited for real-time PCR applications using SYBR Green, and are required for guaranteed qPCR performance of the RT2 qPCR Primer Assays, RT2 Profiler PCR Arrays, RT2 lncRNA qPCR Assays, and RT2 lncRNA PCR Arrays. This Mastermix should be used in qPCR instruments under standard cycling conditions. RT2 SYBR Green qPCR Mastermixes are also suitable for use with EpiTect ChIP and EpiTect Methyl II qPCR Assays and PCR Arrays.
RT2 Easy First Strand Kit
The RT² Easy First Strand Kit provides a rapid and convenient procedure for efficient first strand cDNA synthesis and genomic DNA elimination in RNA samples. Its unique format enables easy parallel processing of multiple RNA samples for reverse transcription. The synthesized cDNA is ready to use in real-time PCR expression analysis of multiple genes when used with RT² SYBR Green Mastermixes, RT² qPCR Primer Assays, or other commercially available primer assays.
RT2 HT First Strand Kit
The RT² HT First Strand Kit provides a rapid and convenient procedure for efficient first-strand cDNA synthesis and genomic DNA elimination in RNA samples. It enables simultaneous and easy parallel processing of 96 RNA samples for reverse transcription. The synthesized cDNA is ready to use in real-time PCR expression analysis of multiple genes.
RT2 First Strand Kit
The RT2 First Strand Kit provides a rapid and convenient procedure for efficient first-strand cDNA synthesis and genomic DNA elimination in RNA samples. The synthesized cDNA is ready to use in real-time PCR expression analysis of multiple genes.
RT2 qPCR Primer Assays
RT² qPCR Primer Assays are specifically designed and experimentally verified for real-time PCR analysis. The rigorous assay verification criteria ensure PCR specificity and efficiency for reliable and accurate gene expression analysis results.